ka22_medium

CultureMech:009835 bacterial COMPLEX LIQUID
Original name: KA22 Medium

Overview

External ontology
TOGO:M449 — KA22 Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M449 Original source: JCM - JCM_M448 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=448

Composition

flowchart LR
    CultureMech_009835["ka22_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009835 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_KH2PO4["KH2PO4"]
    CultureMech_009835 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Resazurin["Resazurin"]
    CultureMech_009835 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_009835 --> ing3_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing3_Na2S_9H2O -.-> CHEBI_76209
    ing4_NaHCO3["NaHCO3"]
    CultureMech_009835 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_009835 --> ing5_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing5_Sulfur__powder_ -.-> CHEBI_33403
    ing6_MES["MES"]
    CultureMech_009835 --> ing6_MES
    CHEBI_39010["CHEBI:39010"]
    ing6_MES -.-> CHEBI_39010
    ing7_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_009835 --> ing7_Sea_salts__Sigma_
    ing8_Mg_NO3__6H2O["Mg(NO3)·6H2O"]
    CultureMech_009835 --> ing8_Mg_NO3__6H2O
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009835 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_N2["N2"]
    CultureMech_009835 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997
    ing11_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009835 --> ing11_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing11_Hydrogen_gas -.-> CHEBI_18276

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
3 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
4 Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Simple component, Defined component
5 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
6 Sulfur (powder) 12 G_PER_L CHEBI:33403
elemental sulfur
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
7 MES 1.95 G_PER_L CHEBI:39010
MES
 Role: Buffer; Properties: Organic compound, Simple component, Defined component
8 Sea salts (Sigma) 30 G_PER_L Role: Mineral source; Properties: Complex component, Defined component
9 Mg(NO3)·6H2O 2.54 G_PER_L Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
12 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス

Solutions

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

Trace vitamins (see Medium [M190])

Dose: 0.5 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T06:35:55.082841Z togo-import Imported from TOGO Medium Source: JCM, ID: M449
2026-01-28T19:48:13.878672Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:22.997690+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:09.275120Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009835
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.249667+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "KA22 Medium" to "ka22_medium" for consistent matching
2026-05-16T09:03:18.115369+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:41.491475+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.668141+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:16.212745+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 7ffe0672306498a39118bc7f488d857a12339b0d8b0b9091d175500bd36248cb