spirochaeta_californiensis_medium

CultureMech:009841 bacterial COMPLEX LIQUID
Original name: Spirochaeta Californiensis Medium

Overview

External ontology
TOGO:M454 — Spirochaeta Californiensis Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M454 Original source: JCM - JCM_M454 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=454

Composition

flowchart LR
    CultureMech_009841["spirochaeta_californiensis_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009841 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_009841 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_009841 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_009841 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_K2HPO4["K2HPO4"]
    CultureMech_009841 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Resazurin["Resazurin"]
    CultureMech_009841 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009841 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009841 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_KCl["KCl"]
    CultureMech_009841 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NaHCO3["NaHCO3"]
    CultureMech_009841 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2CO3["Na2CO3"]
    CultureMech_009841 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_Glucose["Glucose"]
    CultureMech_009841 --> ing11_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing11_Glucose -.-> CHEBI_17234
    ing12_N2["N2"]
    CultureMech_009841 --> ing12_N2
    CHEBI_17997["CHEBI:17997"]
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Yeast extract 0.5 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
5 K2HPO4
HO4P.2K 174.174 g/mol
0.2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.4 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component
9 KCl
Cl.K 74.551 g/mol
0.2 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
10 NaHCO3
CHO3.Na 84.006 g/mol
24 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
11 Na2CO3
CO3.2Na 105.988 g/mol
2.76 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
12 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas

Solutions

Trace vitamins (see Medium [M190])

Dose: 2 G_PER_L

Cross-reference to Medium M190

Trace minerals solution (see Medium [M221])

Dose: 1 G_PER_L

Cross-reference to Medium M221

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.091216Z togo-import Imported from TOGO Medium Source: JCM, ID: M454
2026-01-28T19:48:39.874436Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:05:07.113093+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:20:09.313086Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009841
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:28.286632+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Spirochaeta Californiensis Medium" to "spirochaeta_californiensis_medium" for consistent matching
2026-05-16T09:03:18.146595+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:41.526718+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:50.678604+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:16.254561+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 639f05fb70dbfeb9cf1ed0efac4a53982b882bb47f6a9c9b192968ea23d3e948