Overview
- External ontology
- TOGO:M45 — MGA Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M45 Original source: JCM - JCM_M53 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=53
Composition
flowchart LR
CultureMech_009847["mga_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_009847 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009847 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_009847 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Glucose["Glucose"]
CultureMech_009847 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_L__Asparagine["L--Asparagine"]
CultureMech_009847 --> ing4_L__Asparagine
CHEBI_17196["CHEBI:17196"]
ing4_L__Asparagine -.-> CHEBI_17196
ing5_Agar["Agar"]
CultureMech_009847 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009847 --> ing6_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_7H2O -.-> CHEBI_75836
ing7_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009847 --> ing7_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_7H2O -.-> CHEBI_32312
ing8_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_009847 --> ing8_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing8_CuSO4_5H2O -.-> CHEBI_31440
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 |
L--Asparagine
C4H8N2O3 132.119 g/mol
|
1 G_PER_L |
CHEBI:17196
L-asparagine
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Trace salts solution (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M45_MGA_Agar (CultureMech:009847)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.084557Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M45 |
| 2026-01-28T19:48:24.227368Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.038488+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:09.946901Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:09.350628Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009847 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.320563+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MGA Agar" to "mga_agar" for consistent matching |
| 2026-05-16T09:03:18.173656+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:41.557220+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.671821+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.685381+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.290698+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a077a972259bf7cc3ce2ba22ae8efe7f876feebc956d5bec36a2353f32a6201f |