Overview
- External ontology
- TOGO:M463 — Saltwater Medium With Lactate
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M463 Original source: JCM - JCM_M462 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=462
Composition
flowchart LR
CultureMech_009851["saltwater_medium_with_lactate"]
ing0_Sol__1["Sol. 1"]
CultureMech_009851 --> ing0_Sol__1
ing1_Distilled_water["Distilled water"]
CultureMech_009851 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_NaCl["NaCl"]
CultureMech_009851 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009851 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009851 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009851 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_009851 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009851 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_009851 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Na2SO4["Na2SO4"]
CultureMech_009851 --> ing9_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing9_Na2SO4 -.-> CHEBI_32149
ing10_KBr["KBr"]
CultureMech_009851 --> ing10_KBr
CHEBI_32030["CHEBI:32030"]
ing10_KBr -.-> CHEBI_32030
ing11_Riboflavin__see_below_["Riboflavin (see below)"]
CultureMech_009851 --> ing11_Riboflavin__see_below_
ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009851 --> ing12_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing12_Carbon_dioxide_gas -.-> CHEBI_16526
ing13_Nitrogen_gas["Nitrogen gas"]
CultureMech_009851 --> ing13_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing13_Nitrogen_gas -.-> CHEBI_17997
ing14_Riboflavin["Riboflavin"]
CultureMech_009851 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Sodium_lactate["Sodium lactate"]
CultureMech_009851 --> ing15_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing15_Sodium_lactate -.-> CHEBI_75228
ing16_N2["N2"]
CultureMech_009851 --> ing16_N2
ing16_N2 -.-> CHEBI_17997
ing17_NaHCO3["NaHCO3"]
CultureMech_009851 --> ing17_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing17_NaHCO3 -.-> CHEBI_32139
ing18_Na2S_9H2O["Na2S・9H2O"]
CultureMech_009851 --> ing18_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing18_Na2S_9H2O -.-> CHEBI_76209
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 906 G_PER_L | ||
| 2 |
Distilled water
H2O 18.015 g/mol
|
1141.3 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 900.0, 100.0, 100.0, 31.3, 10.0] |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.75 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
KBr
Br.K 119.002 g/mol
|
0.09 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Riboflavin (see below) | 1 G_PER_L | ||
| 13 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 14 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
2.5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
2.25 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 17 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 18 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 19 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Defined component, Inorganic compound, Simple component |
Solutions
sodium lactate solution
Dose: 100 G_PER_L
NaHCO3 solution
Dose: 31.25 G_PER_L
Na2S・9H2O solution
Dose: 10 G_PER_L
Trace element solution (see Medium [M439])
Dose: 1 G_PER_L
Cross-reference to Medium M439
Vitamin solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Thiamine solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Vitamin B12 solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M463_Saltwater_Medium_With_Lactate (CultureMech:009851)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.104457Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M463 |
| 2026-01-28T19:48:30.644160Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:50.992003+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:09:22.302648Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:20:09.382116Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009851 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.350289+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Saltwater Medium With Lactate" to "saltwater_medium_with_lactate" for consistent matching |
| 2026-05-16T09:03:18.205992+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.590205+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:50.697186+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.328688+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 782686493c2822f53c08dc49b50b374d11a5aa4739195417eafb6f3846a16a3c |