Overview
- External ontology
- TOGO:M467 — Mycobacterium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M467 Original source: JCM - JCM_M466 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=466
Composition
flowchart LR
CultureMech_009855["mycobacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009855 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009855 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_009855 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Tween_80["Tween 80"]
CultureMech_009855 --> ing3_Tween_80
CHEBI_53426["CHEBI:53426"]
ing3_Tween_80 -.-> CHEBI_53426
ing4_Glycerol["Glycerol"]
CultureMech_009855 --> ing4_Glycerol
CHEBI_17754["CHEBI:17754"]
ing4_Glycerol -.-> CHEBI_17754
ing5_Na2HPO4_12H2O["Na2HPO4・12H2O"]
CultureMech_009855 --> ing5_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4_12H2O -.-> CHEBI_34683
ing6_Trisodium_citrate["Trisodium citrate"]
CultureMech_009855 --> ing6_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing6_Trisodium_citrate -.-> CHEBI_53258
ing7_Agar["Agar"]
CultureMech_009855 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009855 --> ing8_Yeast_extract__BD_Difco_
ing9_Proteose_peptone_No__3["Proteose peptone No. 3"]
CultureMech_009855 --> ing9_Proteose_peptone_No__3
ing10_Casein_peptone__tryptic_digested["Casein peptone, tryptic digested"]
CultureMech_009855 --> ing10_Casein_peptone__tryptic_digested
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.5 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 5 |
Glycerol
C3H8O3 92.094 g/mol
|
50 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 6 |
Na2HPO4・12H2O
HO4P.2Na 141.958 g/mol
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
1.5 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 10 | Proteose peptone No. 3 | 2 G_PER_L | Properties: Undefined component | |
| 11 | Casein peptone, tryptic digested | 2 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M467_Mycobacterium_Medium (CultureMech:009855)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.112962Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M467 |
| 2026-01-28T19:48:37.699855Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:09.415811Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009855 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.385778+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mycobacterium Medium" to "mycobacterium_medium" for consistent matching |
| 2026-06-05T07:40:41.627492+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.353791+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:30.721948+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.709414+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.372484+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7c2577c5c37b14b3473f67850a27878ba15f5ad7578aa61bcf2b5b545761e691 |