Overview
- External ontology
- TOGO:M484 — Ethanologenbacterium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M484 Original source: JCM - JCM_M483 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=483
Composition
flowchart LR
CultureMech_009871["ethanologenbacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009871 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_009871 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_K2HPO4["K2HPO4"]
CultureMech_009871 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_009871 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009871 --> ing4_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_7H2O -.-> CHEBI_75836
ing5_Glucose["Glucose"]
CultureMech_009871 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009871 --> ing6_Yeast_extract__BD_Difco_
ing7_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_009871 --> ing7_Tryptone__BD_Difco_
ing8_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_009871 --> ing8_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing9_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
CultureMech_009871 --> ing9_Beef_extract__BD_Difco_
ing10_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009871 --> ing10_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_2H2O -.-> CHEBI_86158
ing11_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009871 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_009871 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009871 --> ing13_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing13_CoCl2_6H2O -.-> CHEBI_35696
ing14_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009871 --> ing14_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_7H2O -.-> CHEBI_32312
ing15_AlK_SO4_2_12H2O["AlK(SO4)2・12H2O"]
CultureMech_009871 --> ing15_AlK_SO4_2_12H2O
CHEBI_86465["CHEBI:86465"]
ing15_AlK_SO4_2_12H2O -.-> CHEBI_86465
ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_009871 --> ing16_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
ing17_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_009871 --> ing17_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing17_MnSO4_xH2O -.-> CHEBI_86360
ing18_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_009871 --> ing18_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_p__Aminobenzoic_acid -.-> CHEBI_30753
ing19_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_009871 --> ing19_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_HCl -.-> CHEBI_30961
ing20_Folic_acid["Folic acid"]
CultureMech_009871 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Riboflavin["Riboflavin"]
CultureMech_009871 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Ascorbic_acid["Ascorbic acid"]
CultureMech_009871 --> ing22_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing22_Ascorbic_acid -.-> CHEBI_22652
ing23_Creatine["Creatine"]
CultureMech_009871 --> ing23_Creatine
CHEBI_16919["CHEBI:16919"]
ing23_Creatine -.-> CHEBI_16919
ing24_Cyanocobalamin["Cyanocobalamin"]
CultureMech_009871 --> ing24_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing24_Cyanocobalamin -.-> CHEBI_17439
ing25_Citric_acid["Citric acid"]
CultureMech_009871 --> ing25_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing25_Citric_acid -.-> CHEBI_30769
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 7 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 8 | Tryptone (BD-Difco) | 4 G_PER_L | Properties: Undefined component | |
| 9 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 10 | Beef extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 11 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.05 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
AlK(SO4)2・12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.01 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
4.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 18 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.01 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
0.05 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.01 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.025 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 | Ascorbic acid | 0.025 G_PER_L |
CHEBI:22652
ascorbic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Creatine
C4H9N3O2 131.135 g/mol
|
0.025 G_PER_L |
CHEBI:16919
creatine
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 25 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.01 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin |
| 26 |
Citric acid
C6H8O7 192.123 g/mol
|
0.02 G_PER_L |
CHEBI:30769
citric acid
|
Properties: Organic compound, Simple component, Defined component |
Solutions
Microelement solution (see below)
Dose: 10 G_PER_L
Vitamin solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M484_Ethanologenbacterium_Medium (CultureMech:009871)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.140485Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M484 |
| 2026-01-28T19:48:31.906168Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:53.124938+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:24.564484Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:09.542697Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009871 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.508515+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ethanologenbacterium Medium" to "ethanologenbacterium_medium" for consistent matching |
| 2026-05-16T09:03:18.374973+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:41.779655+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.398133+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:50.751245+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.534407+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3ac9db3471aa5fa6b85980a57d53fad9dbf6fa8c89bfdc2b9f8eada94d6be27a |