Overview
- External ontology
- TOGO:M493 — MMJ-TL Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M493 Original source: JCM - JCM_M492 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=492
Composition
flowchart LR
CultureMech_009881["mmj_tl_medium"]
ing0_Resazurin["Resazurin"]
CultureMech_009881 --> ing0_Resazurin
CHEBI_8806["CHEBI:8806"]
ing0_Resazurin -.-> CHEBI_8806
ing1_Sulfur__powder_["Sulfur (powder)"]
CultureMech_009881 --> ing1_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing1_Sulfur__powder_ -.-> CHEBI_33403
ing2_Modified_MJ_synthetic_seawater__see_below_["Modified MJ synthetic seawater (see below)"]
CultureMech_009881 --> ing2_Modified_MJ_synthetic_seawater__see_below_
ing3_NaOH["NaOH"]
CultureMech_009881 --> ing3_NaOH
CHEBI_32145["CHEBI:32145"]
ing3_NaOH -.-> CHEBI_32145
ing4_N2["N2"]
CultureMech_009881 --> ing4_N2
CHEBI_17997["CHEBI:17997"]
ing4_N2 -.-> CHEBI_17997
ing5_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009881 --> ing5_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing5_Carbon_dioxide_gas -.-> CHEBI_16526
ing6_Hydrogen_gas["Hydrogen gas"]
CultureMech_009881 --> ing6_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing6_Hydrogen_gas -.-> CHEBI_18276
ing7_Distilled_water["Distilled water"]
CultureMech_009881 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
ing8_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_009881 --> ing8_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_7H2O -.-> CHEBI_31795
ing9_NaCl["NaCl"]
CultureMech_009881 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009881 --> ing10_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_2H2O -.-> CHEBI_86158
ing11_NH4Cl["NH4Cl"]
CultureMech_009881 --> ing11_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing11_NH4Cl -.-> CHEBI_31206
ing12_K2HPO4["K2HPO4"]
CultureMech_009881 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
ing13_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_009881 --> ing13_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing13_MgCl2_6H2O -.-> CHEBI_86345
ing14_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_009881 --> ing14_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_7H2O -.-> CHEBI_75836
ing15_KCl["KCl"]
CultureMech_009881 --> ing15_KCl
CHEBI_32588["CHEBI:32588"]
ing15_KCl -.-> CHEBI_32588
ing16_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_009881 --> ing16_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_6H2O -.-> CHEBI_34887
ing17_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_009881 --> ing17_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing17_Na2SeO3_5H2O -.-> CHEBI_131361
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 2 | Sulfur (powder) | 3 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Modified MJ synthetic seawater (see below) | 1 G_PER_L | ||
| 4 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 5 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 6 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 7 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
| 8 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 14 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.18 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 15 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 16 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 17 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
1 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 18 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
1 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
Solutions
1.0 M Sodium acetate solution
Dose: 10 G_PER_L
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
1% Yeast extract solution
Dose: 10 G_PER_L
Role: Nutrient source; Properties: Undefined component
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
5% Na2S·9H2O solution
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Inorganic compound, Solution, Defined component
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M493_MMJ-TL_Medium (CultureMech:009881)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.158435Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M493 |
| 2026-01-28T19:48:42.638633Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:11.855173+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:09:43.696808Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:09.616836Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009881 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.579991+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ-TL Medium" to "mmj_tl_medium" for consistent matching |
| 2026-05-16T09:03:18.457455+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.881935+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:30.919784+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.771751+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.617393+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d9a501437008d5f16de0d5649f5b6a08cae82e1c278bc5516d8976734832114d |