Overview
- External ontology
- TOGO:M496 — MMJ-DO Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M496 Original source: JCM - JCM_M495 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=495
Composition
flowchart LR
CultureMech_009884["mmj_do_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_009884 --> ing0_Yeast_extract
ing1_Resazurin["Resazurin"]
CultureMech_009884 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_NaHCO3["NaHCO3"]
CultureMech_009884 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_009884 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_009884 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_Sodium_ascorbate["Sodium ascorbate"]
CultureMech_009884 --> ing5_Sodium_ascorbate
CHEBI_113451["CHEBI:113451"]
ing5_Sodium_ascorbate -.-> CHEBI_113451
ing6_NaOH["NaOH"]
CultureMech_009884 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_009884 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Hydrogen_gas["Hydrogen gas"]
CultureMech_009884 --> ing8_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing8_Hydrogen_gas -.-> CHEBI_18276
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.5 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 6 |
Sodium ascorbate
C6H7O6.Na 198.106 g/mol
|
0.1 G_PER_L |
CHEBI:113451
sodium ascorbate
|
Properties: Defined component, Organic compound, Simple component |
| 7 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 9 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: Gas |
Solutions
Modified MJ synthetic seawater (see Medium [M493])
Dose: 1 G_PER_L
Cross-reference to Medium M493
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M496_MMJ-DO_Medium (CultureMech:009884)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.163230Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M496 |
| 2026-01-28T19:48:15.740398Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:25.935510+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:09.638306Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009884 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.600726+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ-DO Medium" to "mmj_do_medium" for consistent matching |
| 2026-05-16T09:03:18.481219+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:41.905926+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:16.646946+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6a2c878eb667acea0430978996f7f8ec61ab631ffa4849312bdbc80f5568ebb1 |