Overview
- External ontology
- TOGO:M507 — Ignisphaera Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M507 Original source: JCM - JCM_M506 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=506
Composition
flowchart LR
CultureMech_009897["ignisphaera_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009897 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009897 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_009897 --> ing2_Yeast_extract
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009897 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009897 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_009897 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_009897 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_L__Cystine["L--Cystine"]
CultureMech_009897 --> ing7_L__Cystine
ing8_Soluble_starch["Soluble starch"]
CultureMech_009897 --> ing8_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing8_Soluble_starch -.-> CHEBI_28017
ing9_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_009897 --> ing9_Trypticase_peptone__BD_BBL_
ing10_N2["N2"]
CultureMech_009897 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009897 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_009897 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009897 --> ing13_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_7H2O -.-> CHEBI_32312
ing14_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_009897 --> ing14_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_5H2O -.-> CHEBI_31440
ing15_CoSO4_7H2O["CoSO4・7H2O"]
CultureMech_009897 --> ing15_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_7H2O -.-> CHEBI_91244
ing16_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_009897 --> ing16_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing16_MnSO4_xH2O -.-> CHEBI_86360
ing17_H2SO4["H2SO4"]
CultureMech_009897 --> ing17_H2SO4
CHEBI_26836["CHEBI:26836"]
ing17_H2SO4 -.-> CHEBI_26836
ing18_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_009897 --> ing18_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing18_FeCl3_6H2O -.-> CHEBI_86254
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.28 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.074 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | L--Cystine | 0.3 G_PER_L | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component | |
| 9 | Soluble starch | 2 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 10 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component | |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 12 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.025 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.5 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.016 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoSO4・7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.046 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
2.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
H2SO4
H2O4S 98.079 g/mol
|
0.5 G_PER_L |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Solution |
| 19 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.28 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace elements (see below)
Dose: 1 G_PER_L
FeCl3 solution (see below)
Dose: 1 G_PER_L
5% Na2S・9H2O solution (adjusted pH to 7.0)
Dose: 6 G_PER_L
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M507_Ignisphaera_Medium (CultureMech:009897)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.189808Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M507 |
| 2026-01-28T19:48:24.192440Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:39.981420+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:09.889171Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:09.760098Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009897 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.713689+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ignisphaera Medium" to "ignisphaera_medium" for consistent matching |
| 2026-05-16T09:03:18.571418+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.002110+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.013611+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.808854+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:27.542114+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4267364648de172d86e4a9d75dfd541ca73eb59dfaa6379a7def32ab929c4d7a |