Overview
- External ontology
- TOGO:M511 — NMS Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M511 Original source: JCM - JCM_M510 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=510
Composition
flowchart LR
CultureMech_009902["nms_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009902 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009902 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009902 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KNO3["KNO3"]
CultureMech_009902 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_Ferric_citrate["Ferric citrate"]
CultureMech_009902 --> ing4_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing4_Ferric_citrate -.-> CHEBI_144421
ing5_methane__air__50_50_["methane--air (50:50)"]
CultureMech_009902 --> ing5_methane__air__50_50_
ing6_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009902 --> ing6_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_2H2O -.-> CHEBI_75213
ing7_H3BO3["H3BO3"]
CultureMech_009902 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_009902 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_009902 --> ing9_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_4H2O -.-> CHEBI_86368
ing10_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009902 --> ing10_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing10_CoCl2_6H2O -.-> CHEBI_35696
ing11_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009902 --> ing11_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_6H2O -.-> CHEBI_34887
ing12_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_009902 --> ing12_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_7H2O -.-> CHEBI_32312
ing13_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_009902 --> ing13_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_2H2O -.-> CHEBI_86318
ing14_EDTA_2Na["EDTA・2Na"]
CultureMech_009902 --> ing14_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing14_EDTA_2Na -.-> CHEBI_64734
ing15_KH2PO4["KH2PO4"]
CultureMech_009902 --> ing15_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing15_KH2PO4 -.-> CHEBI_63036
ing16_Na2HPO4_12H2O["Na2HPO4・12H2O"]
CultureMech_009902 --> ing16_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing16_Na2HPO4_12H2O -.-> CHEBI_34683
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.13 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
4 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 6 | methane--air (50:50) | variable VARIABLE | Properties: Gas | |
| 7 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
30 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
200 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
3 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
20 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
10 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
1 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
EDTA・2Na
C10H14N2O8.2Na 336.208 g/mol
|
500 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 16 |
KH2PO4
H2O4P.K 136.084 g/mol
|
5.44 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2HPO4・12H2O
HO4P.2Na 141.958 g/mol
|
14.3 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace element solution (see below)
Dose: 0.5 G_PER_L
20 x Phosphate solution (see below)
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M511_NMS_Medium (CultureMech:009902)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.199476Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M511 |
| 2026-01-28T19:48:17.008890Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: methane--air (50:50) |
| 2026-03-09T08:04:28.128493+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:56.885332Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:09.805206Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009902 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.757335+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NMS Medium" to "nms_medium" for consistent matching |
| 2026-05-16T09:03:18.619778+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.051911+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.059816+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.828054+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:16.793730+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 30a7d3c988d4f05f88dc56819ca488e829c7fdc18d1fd5257e4e9b6779b0ff44 |