Overview
- External ontology
- TOGO:M538 — PHE91 Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M538 Original source: JCM - JCM_M536 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=536
Composition
flowchart LR
CultureMech_009930["phe91_medium"]
ing0_N2["N2"]
CultureMech_009930 --> ing0_N2
CHEBI_17997["CHEBI:17997"]
ing0_N2 -.-> CHEBI_17997
ing1_Distilled_water["Distilled water"]
CultureMech_009930 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009930 --> ing2_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_7H2O -.-> CHEBI_31795
ing3_NH4H2PO4["NH4H2PO4"]
CultureMech_009930 --> ing3_NH4H2PO4
CHEBI_62982["CHEBI:62982"]
ing3_NH4H2PO4 -.-> CHEBI_62982
ing4_25__HCl__7_7_M_["25% HCl (7.7 M)"]
CultureMech_009930 --> ing4_25__HCl__7_7_M_
ing5_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009930 --> ing5_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_2H2O -.-> CHEBI_75213
ing6_H3BO3["H3BO3"]
CultureMech_009930 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_009930 --> ing7_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_4H2O -.-> CHEBI_86368
ing8_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009930 --> ing8_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing8_CoCl2_6H2O -.-> CHEBI_35696
ing9_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009930 --> ing9_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing9_NiCl2_6H2O -.-> CHEBI_34887
ing10_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_009930 --> ing10_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_2H2O -.-> CHEBI_86318
ing11_ZnCl2["ZnCl2"]
CultureMech_009930 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_009930 --> ing12_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_4H2O -.-> CHEBI_86249
ing13_Biotin["Biotin"]
CultureMech_009930 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_009930 --> ing14_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing14_p__Aminobenzoic_acid -.-> CHEBI_30753
ing15_Thiamine_HCl["Thiamine・HCl"]
CultureMech_009930 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
ing16_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_009930 --> ing16_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_HCl -.-> CHEBI_30961
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_009930 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_DL__Calcium_pantothenate["DL--Calcium pantothenate"]
CultureMech_009930 --> ing18_DL__Calcium_pantothenate
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 2 |
Distilled water
H2O 18.015 g/mol
|
971.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 969.0, 1.0, 1.0] |
| 3 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.49 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4H2PO4
H2O4P.H4N 115.025 g/mol
|
0.46 G_PER_L |
CHEBI:62982
ammonium dihydrogen phosphate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 | 25% HCl (7.7 M) | 10 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 6 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
62 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
17 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2.5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
12.5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
12.5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
62.5 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
25 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 19 | DL--Calcium pantothenate | 6.5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
CaCl2・2H2O solution (68 mM)
Dose: 1 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
Pyruvate solution (1 M)
Dose: 10 G_PER_L
Role: Carbon source; Properties: Solution, Defined component, Organic compound
Resazurin solution (0.02%)
Dose: 1 G_PER_L
Role: pH dependent redox indicator; Properties: Solution, Defined component
K2HPO4 solution (609 mM)
Dose: 10 G_PER_L
Role: Buffer; Properties: Defined component, Solution, Inorganic compound
Dithionite solution (0.2 M)
Dose: 5 G_PER_L
Properties: Defined component, Inorganic compound, Solution
Basal salt solution (see below)
Dose: 969 G_PER_L
SL--7 trace element solution (see below)
Dose: 1 G_PER_L
Vitamin solution (see below)
Dose: 1 G_PER_L
Vitamin B12 solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M538_PHE91_Medium (CultureMech:009930)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.250410Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M538 |
| 2026-01-28T19:48:41.970285Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:05:10.738137+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 10 solution(s) from ingredients |
| 2026-03-13T03:09:42.540604Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:10.033519Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009930 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:28.973391+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PHE91 Medium" to "phe91_medium" for consistent matching |
| 2026-05-16T09:03:18.858238+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.294167+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.517171+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:31.270656+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.910357+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:17.039665+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7505d03095d68b53dad894b613590e07b04fe77212615a5bcfbaa93416377699 |