Overview
- External ontology
- TOGO:M557 — Sulfate Reducing Bacterium Medium With Pyruvate
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M557 Original source: JCM - JCM_M553-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=553
Composition
flowchart LR
CultureMech_009951["sulfate_reducing_bacterium_medium_with_pyruvate"]
ing0_Distilled_water["Distilled water"]
CultureMech_009951 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009951 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_009951 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009951 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009951 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_009951 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_009951 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Na2SO4["Na2SO4"]
CultureMech_009951 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_sodium_pyruvate["sodium pyruvate"]
CultureMech_009951 --> ing8_sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing8_sodium_pyruvate -.-> CHEBI_50144
ing9_agar__BD_Difco_["agar (BD-Difco)"]
CultureMech_009951 --> ing9_agar__BD_Difco_
ing10_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_009951 --> ing10_Yeast_extract__BD_Difco_
ing11_L__Cysteine_H2O_HCl["L--Cysteine・H2O・HCl"]
CultureMech_009951 --> ing11_L__Cysteine_H2O_HCl
ing12_NaOH["NaOH"]
CultureMech_009951 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
ing13_N2["N2"]
CultureMech_009951 --> ing13_N2
CHEBI_17997["CHEBI:17997"]
ing13_N2 -.-> CHEBI_17997
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1.9 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 10 | agar (BD-Difco) | 15 G_PER_L | Role: Solidifying component | |
| 11 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 12 | L--Cysteine・H2O・HCl | 0.5 G_PER_L | Properties: Defined component, Simple component, Organic compound | |
| 13 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 14 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Vitamin solution (see Medium [M554])
Dose: 1 G_PER_L
Cross-reference to Medium M554
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M557_Sulfate_Reducing_Bacterium_Medium_With_Pyruvate (CultureMech:009951)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.294867Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M557 |
| 2026-01-28T19:48:15.175528Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:25.278772+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:10.236314Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009951 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.166413+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Sulfate Reducing Bacterium Medium With Pyruvate" to "sulfate_reducing_bacterium_medium_with_pyruvate" for consistent matching |
| 2026-05-16T09:03:19.054211+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.502018+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.427828+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:50.993129+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:17.288904+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9fafa0a147d91880b5068333cd4754cb03d83853427c84f6c0f2a42a8b8e5c64 |