1_2_sme_medium

CultureMech:009958 bacterial COMPLEX LIQUID
Original name: 1/2 SME Medium

Overview

External ontology
TOGO:M563 — 1/2 SME Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M563 Original source: JCM - JCM_M559 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=559

Composition

flowchart LR
    CultureMech_009958["1_2_sme_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_009958 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_009958 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_009958 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_009958 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_009958 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Resazurin["Resazurin"]
    CultureMech_009958 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_009958 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_009958 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_H3BO3["H3BO3"]
    CultureMech_009958 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_KCl["KCl"]
    CultureMech_009958 --> ing9_KCl
    CHEBI_32588["CHEBI:32588"]
    ing9_KCl -.-> CHEBI_32588
    ing10_NaHCO3["NaHCO3"]
    CultureMech_009958 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11__NH4_2SO4["(NH4)2SO4"]
    CultureMech_009958 --> ing11__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing11__NH4_2SO4 -.-> CHEBI_62946
    ing12_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_009958 --> ing12_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing12_Sulfur__powder_ -.-> CHEBI_33403
    ing13_NaBr["NaBr"]
    CultureMech_009958 --> ing13_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing13_NaBr -.-> CHEBI_63004
    ing14_SrCl2_6H2O["SrCl2・6H2O"]
    CultureMech_009958 --> ing14_SrCl2_6H2O
    CHEBI_36385["CHEBI:36385"]
    ing14_SrCl2_6H2O -.-> CHEBI_36385
    ing15_KI["KI"]
    CultureMech_009958 --> ing15_KI
    CHEBI_8346["CHEBI:8346"]
    ing15_KI -.-> CHEBI_8346
    ing16_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_009958 --> ing16_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing16_Carbon_dioxide_gas -.-> CHEBI_16526
    ing17_Nitrogen_gas["Nitrogen gas"]
    CultureMech_009958 --> ing17_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing17_Nitrogen_gas -.-> CHEBI_17997
    ing18_Hydrogen_gas["Hydrogen gas"]
    CultureMech_009958 --> ing18_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing18_Hydrogen_gas -.-> CHEBI_18276

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
3.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 NaCl
Cl.Na 58.443 g/mol
13.85 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.38 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
2.75 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
9 H3BO3
H3BO3 61.833 g/mol
0.015 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 NaHCO3
CHO3.Na 84.006 g/mol
0.16 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
12 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.25 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
13 Sulfur (powder) 10 G_PER_L CHEBI:33403
elemental sulfur
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 NaBr
Br.Na 102.894 g/mol
0.05 G_PER_L CHEBI:63004
sodium bromide
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
15 SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
7.5 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16 KI
I.K 166.002 g/mol
0.05 G_PER_L CHEBI:8346
potassium iodide
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
18 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
19 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T06:35:55.310110Z togo-import Imported from TOGO Medium Source: JCM, ID: M563
2026-01-28T19:48:29.220993Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas
2026-03-13T06:20:10.301956Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:009958
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:29.232120+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/2 SME Medium" to "1_2_sme_medium" for consistent matching
2026-05-16T09:03:19.127750+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:42.580192+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:31.484529+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.020639+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:17.378546+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 5be55e136801cfd5d33e02e58c9b18c3b5856401d9500a9863aa69e7e04f943b