Overview
- External ontology
- TOGO:M572 — Roseospira Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M572 Original source: JCM - JCM_M568 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=568
Composition
flowchart LR
CultureMech_009968["roseospira_medium"]
ing0_Ferric_citrate__0_1___w_v_["Ferric citrate (0.1%, w/v)"]
CultureMech_009968 --> ing0_Ferric_citrate__0_1___w_v_
ing1_Distilled_water["Distilled water"]
CultureMech_009968 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_009968 --> ing2_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_7H2O -.-> CHEBI_31795
ing3_Yeast_extract["Yeast extract"]
CultureMech_009968 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_009968 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_009968 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_KH2PO4["KH2PO4"]
CultureMech_009968 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NH4Cl["NH4Cl"]
CultureMech_009968 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_009968 --> ing8_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing8_Sodium_pyruvate -.-> CHEBI_50144
ing9_NaHCO3["NaHCO3"]
CultureMech_009968 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Na2S2O3["Na2S2O3"]
CultureMech_009968 --> ing10_Na2S2O3
CHEBI_32150["CHEBI:32150"]
ing10_Na2S2O3 -.-> CHEBI_32150
ing11_HCl__25___v_v_["HCl (25%, v/v)"]
CultureMech_009968 --> ing11_HCl__25___v_v_
ing12_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_009968 --> ing12_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_009968 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_009968 --> ing14_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_4H2O -.-> CHEBI_86368
ing15_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_009968 --> ing15_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2_6H2O -.-> CHEBI_35696
ing16_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_009968 --> ing16_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_6H2O -.-> CHEBI_34887
ing17_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_009968 --> ing17_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_2H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_009968 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Ferric citrate (0.1%, w/v) | 5 G_PER_L | Role: Mineral source; Properties: Defined component, Organic compound, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 3 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 | Yeast extract | 0.4 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.6 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
variable VARIABLE |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Solution |
| 11 |
Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
|
variable VARIABLE |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Solution |
| 12 | HCl (25%, v/v) | 1 G_PER_L | Role: Solvating media; Properties: Solution, Inorganic compound, Defined component | |
| 13 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
40 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
60 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
200 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
20 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
20 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Micronutrient solution (SL7) (see below)
Dose: 1 G_PER_L
vitamin B12 solution
Dose: 1 G_PER_L
See mediadive_6105_Vitamin_B12_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M572_Roseospira_Medium (CultureMech:009968)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.328060Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M572 |
| 2026-01-28T19:48:12.077969Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaHCO3; Added default concentration for ingredient: Na2S2O3 |
| 2026-03-09T08:04:19.919949+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:47.854918Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:10.380701Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009968 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.310327+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Roseospira Medium" to "roseospira_medium" for consistent matching |
| 2026-05-16T09:03:19.212762+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.657550+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:31.563333+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.053034+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:17.472863+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5ad323997de47fb89b8cf4d65cd4bdae63c74a1af62ab0d65525eefb04a5c0f0 |