Overview
- External ontology
- TOGO:M595 — A-MMX Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M595 Original source: JCM - JCM_M590 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=590
Composition
flowchart LR
CultureMech_009992["a_mmx_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_009992 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_009992 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_009992 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_009992 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_009992 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_009992 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Na2S_9H2O["Na2S·9H2O"]
CultureMech_009992 --> ing6_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_9H2O -.-> CHEBI_76209
ing7_Peptone["Peptone"]
CultureMech_009992 --> ing7_Peptone
ing8_N2["N2"]
CultureMech_009992 --> ing8_N2
CHEBI_17997["CHEBI:17997"]
ing8_N2 -.-> CHEBI_17997
ing9_Soluble_starch["Soluble starch"]
CultureMech_009992 --> ing9_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing9_Soluble_starch -.-> CHEBI_28017
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
875.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 775.0, 100.0] |
| 2 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
113 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.19 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 7 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Simple component, Defined component, Inorganic compound |
| 8 | Peptone | 0.2 G_PER_L | ||
| 9 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 10 | Soluble starch | 5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
Solutions
Synthetic seawater (2 x) (see Medium [M236])
Dose: 125 G_PER_L
Cross-reference to Medium M236
Starch solution (see below)
Dose: 100 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M595_A-MMX_Medium (CultureMech:009992)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.368370Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M595 |
| 2026-01-28T19:48:31.846505Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:53.034021+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:24.468083Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:10.559937Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:009992 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.486916+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "A-MMX Medium" to "a_mmx_medium" for consistent matching |
| 2026-05-16T09:03:19.375272+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:42.829019+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.104298+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:17.634766+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b5a6d8969976b4de43cfb9c569459c56290cca05f092b9ad53be3631e9ec5c4a |