Overview
- External ontology
- TOGO:M644 — MMB Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M644 Original source: JCM - JCM_M631 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=631
Composition
flowchart LR
CultureMech_010045["mmb_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010045 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010045 --> ing1_Yeast_extract
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_010045 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_Glucose["Glucose"]
CultureMech_010045 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Peptone["Peptone"]
CultureMech_010045 --> ing4_Peptone
ing5_Hunter_s_basal_salts__see_below_["Hunter's basal salts (see below)"]
CultureMech_010045 --> ing5_Hunter_s_basal_salts__see_below_
ing6_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010045 --> ing6_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_7H2O -.-> CHEBI_31795
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010045 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010045 --> ing8_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_7H2O -.-> CHEBI_75836
ing9_Biotin["Biotin"]
CultureMech_010045 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Thiamine_HCl["Thiamine・HCl"]
CultureMech_010045 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_Nicotinic_acid["Nicotinic acid"]
CultureMech_010045 --> ing11_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing11_Nicotinic_acid -.-> CHEBI_15940
ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_010045 --> ing12_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
ing13__NH4_6Mo7O24_4H2O["(NH4)6Mo7O24・4H2O"]
CultureMech_010045 --> ing13__NH4_6Mo7O24_4H2O
CHEBI_91249["CHEBI:91249"]
ing13__NH4_6Mo7O24_4H2O -.-> CHEBI_91249
ing14_KOH["KOH"]
CultureMech_010045 --> ing14_KOH
CHEBI_32035["CHEBI:32035"]
ing14_KOH -.-> CHEBI_32035
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
951.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 950.0] |
| 2 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 5 | Peptone | 0.1 G_PER_L | ||
| 6 | Hunter's basal salts (see below) | 20 G_PER_L | ||
| 7 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
14.45 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.42 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
99 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 11 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
25 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 12 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
50 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
| 13 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 14 |
(NH4)6Mo7O24・4H2O
2H4N.MoO4 196.014 g/mol
|
9.25 G_PER_L |
CHEBI:91249
ammonium molybdate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 15 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Metals ''44'' (see Medium [M140])
Dose: 50 G_PER_L
Cross-reference to Medium M140
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M644_MMB_Medium (CultureMech:010045)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.454902Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M644 |
| 2026-01-28T19:48:22.969917Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:38.176447+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:07.943917Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:10.922521Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010045 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:29.850232+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMB Medium" to "mmb_medium" for consistent matching |
| 2026-05-16T09:03:19.726982+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.216844+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.037439+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.223671+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:18.039592+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bc65708bd616fb46473c8ffbabb269475c2532f802bf131e9e9dd187a5e6da85 |