Overview
- External ontology
- TOGO:M689 — Spirochaeta Caldaria Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M689 Original source: JCM - JCM_M670 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=670
Composition
flowchart LR
CultureMech_010094["spirochaeta_caldaria_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010094 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Resazurin["Resazurin"]
CultureMech_010094 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_Glucose["Glucose"]
CultureMech_010094 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010094 --> ing3_Tryptone__BD_Difco_
ing4_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_010094 --> ing4_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing5_N2["N2"]
CultureMech_010094 --> ing5_N2
CHEBI_17997["CHEBI:17997"]
ing5_N2 -.-> CHEBI_17997
ing6_NaOH["NaOH"]
CultureMech_010094 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 4 | Tryptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 5 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.25 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 6 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 7 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Solutions
FeCl2 solution (see Medium [M180])
Dose: 10 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 10 G_PER_L
Cross-reference to Medium M180
Trace minerals (see Medium [M142])
Dose: 25 G_PER_L
Cross-reference to Medium M142
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M689_Spirochaeta_Caldaria_Medium (CultureMech:010094)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.530487Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M689 |
| 2026-01-28T19:48:20.152398Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:33.418082+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:20:11.245911Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010094 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.174113+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Spirochaeta Caldaria Medium" to "spirochaeta_caldaria_medium" for consistent matching |
| 2026-05-16T09:03:20.043601+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:43.562348+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:18.420408+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9cc12b9c33e602b60db03b641abda3ca89672c6c353dce4a40935a4fcc13683e |