sporosalibacterium_medium

CultureMech:010136 bacterial COMPLEX LIQUID
Original name: Sporosalibacterium Medium

Overview

External ontology
TOGO:M728 — Sporosalibacterium Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M728 Original source: JCM - JCM_M706 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=706

Composition

flowchart LR
    CultureMech_010136["sporosalibacterium_medium"]
    ing0_Sol__1["Sol. 1"]
    CultureMech_010136 --> ing0_Sol__1
    ing1_Sol__2["Sol. 2"]
    CultureMech_010136 --> ing1_Sol__2
    ing2_Distilled_water["Distilled water"]
    CultureMech_010136 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_010136 --> ing3_Yeast_extract
    ing4_NaCl["NaCl"]
    CultureMech_010136 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010136 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_KH2PO4["KH2PO4"]
    CultureMech_010136 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_NH4Cl["NH4Cl"]
    CultureMech_010136 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_K2HPO4["K2HPO4"]
    CultureMech_010136 --> ing8_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing8_K2HPO4 -.-> CHEBI_131527
    ing9_Resazurin["Resazurin"]
    CultureMech_010136 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Glucose["Glucose"]
    CultureMech_010136 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_010136 --> ing11_Trypticase_peptone__BD_BBL_
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010136 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010136 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997
    ing14_5__L__Cysteine_HCl_H2O["5% L--Cysteine・HCl・H2O"]
    CultureMech_010136 --> ing14_5__L__Cysteine_HCl_H2O

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Sol. 1 4.7 G_PER_L
2 Sol. 2 0.39 G_PER_L
3 Distilled water
H2O 18.015 g/mol
940 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
4 Yeast extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
5 NaCl
Cl.Na 58.443 g/mol
40 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
7 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
8 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
9 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
10 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
11 Glucose
C6H12O6 180.156 g/mol
3.6 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
12 Trypticase peptone (BD-BBL) 2 G_PER_L Properties: Undefined component
13 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
14 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
15 5% L--Cysteine・HCl・H2O 0.05 G_PER_L Properties: Defined component, Organic compound, Solution

Solutions

Trace element solution (see Medium [M439])

Dose: 1 G_PER_L

Cross-reference to Medium M439

5% NaHCO3 solution

Dose: 0.2 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

15% MgCl2・6H2O solution

Dose: 0.1 G_PER_L

Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

5% Na2S・9H2O solution

Dose: 0.04 G_PER_L

Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.612407Z togo-import Imported from TOGO Medium Source: JCM, ID: M728
2026-01-28T19:48:15.441614Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:25.516291+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T06:20:11.605154Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010136
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.536209+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Sporosalibacterium Medium" to "sporosalibacterium_medium" for consistent matching
2026-05-16T09:03:20.442527+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:43.962168+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.487637+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:18.772843+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 454959b35612b3d3d2e15f8fcbef5211f118f29b7222dc994eb10471ad6c53db