anaerobic_marine_broth_2216

CultureMech:010147 bacterial COMPLEX LIQUID
Original name: Anaerobic Marine Broth 2216

Overview

External ontology
TOGO:M742 — Anaerobic Marine Broth 2216
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M742 Original source: JCM - JCM_M719 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=719

Composition

flowchart LR
    CultureMech_010147["anaerobic_marine_broth_2216"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010147 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Resazurin["Resazurin"]
    CultureMech_010147 --> ing1_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Resazurin -.-> CHEBI_8806
    ing2_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_010147 --> ing2_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing2_Na2S_9H2O -.-> CHEBI_76209
    ing3_Marine_broth_2216__BD_Difco_["Marine broth 2216 (BD-Difco)"]
    CultureMech_010147 --> ing3_Marine_broth_2216__BD_Difco_
    ing4_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010147 --> ing4_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing5_N2["N2"]
    CultureMech_010147 --> ing5_N2
    CHEBI_17997["CHEBI:17997"]
    ing5_N2 -.-> CHEBI_17997

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
3 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
Role: Reducing agent; Properties: Simple component, Inorganic compound, Defined component
4 Marine broth 2216 (BD-Difco) 37.4 G_PER_L Properties: Undefined component, Complex component
5 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
Properties: Organic compound, Simple component, Defined component
6 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: Gas

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.631713Z togo-import Imported from TOGO Medium Source: JCM, ID: M742
2026-01-28T19:48:08.346398Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-13T06:20:11.684103Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010147
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.614849+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Anaerobic Marine Broth 2216" to "anaerobic_marine_broth_2216" for consistent matching
2026-05-16T09:03:24.345531+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:48.911035+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:23.440639+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 1a904c14a5f5d3ca2d5177a7d5a7cb7d630463cb7b4ba76a66342af3ceb16129