Overview
- External ontology
- TOGO:M747 — Ravot Modified Medium For Marinitoga Litoralis
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M747 Original source: JCM - JCM_M724 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=724
Composition
flowchart LR
CultureMech_010152["ravot_modified_medium_for_marinitoga_litoralis"]
ing0_Distilled_water["Distilled water"]
CultureMech_010152 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010152 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_010152 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010152 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010152 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_010152 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_010152 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010152 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_010152 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Sulfur__powder_["Sulfur (powder)"]
CultureMech_010152 --> ing9_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing9_Sulfur__powder_ -.-> CHEBI_33403
ing10_Maltose["Maltose"]
CultureMech_010152 --> ing10_Maltose
CHEBI_18167["CHEBI:18167"]
ing10_Maltose -.-> CHEBI_18167
ing11_PIPES["PIPES"]
CultureMech_010152 --> ing11_PIPES
CHEBI_39033["CHEBI:39033"]
ing11_PIPES -.-> CHEBI_39033
ing12_Sodium_acetate_3H2O["Sodium acetate・3H2O"]
CultureMech_010152 --> ing12_Sodium_acetate_3H2O
CHEBI_32954["CHEBI:32954"]
ing12_Sodium_acetate_3H2O -.-> CHEBI_32954
ing13_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_010152 --> ing13_Trypticase_peptone__BD_BBL_
ing14_N2["N2"]
CultureMech_010152 --> ing14_N2
CHEBI_17997["CHEBI:17997"]
ing14_N2 -.-> CHEBI_17997
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
26 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 10 | Sulfur (powder) | 5 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 11 |
Maltose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 12 | PIPES | 3.3 G_PER_L |
CHEBI:39033
PIPES
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 13 |
Sodium acetate・3H2O
C2H3O2.Na 82.034 g/mol
|
0.83 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 14 | Trypticase peptone (BD-BBL) | 2 G_PER_L | Properties: Undefined component | |
| 15 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
7% K2HPO4solution
Dose: 4.3 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
7% KH2PO4 solution
Dose: 4.3 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
3% Na2S・9H2O solution
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M747_Ravot_Modified_Medium_For_Marinitoga_Litoralis (CultureMech:010152)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.639374Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M747 |
| 2026-01-28T19:48:27.259761Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:45.189754+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:15.557403Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.716961Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010152 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.647001+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ravot Modified Medium For Marinitoga Litoralis" to "ravot_modified_medium_for_marinitoga_litoralis" for consistent matching |
| 2026-05-16T09:03:20.548839+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.080448+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.523981+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:18.920562+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e9ff2c12e08804e0876eca9d60a81ca5fc93be44a108bc39408cc0e957884707 |