Overview
- External ontology
- TOGO:M761 — 1/2 R2A Medium With 75% Artificial Seawater
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M761 Original source: JCM - JCM_M736 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=736
Composition
flowchart LR
CultureMech_010168["1_2_r2a_medium_with_75_artificial_seawater"]
ing0_Distilled_water["Distilled water"]
CultureMech_010168 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_010168 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_010168 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_010168 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_Glucose["Glucose"]
CultureMech_010168 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Soluble_starch["Soluble starch"]
CultureMech_010168 --> ing5_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing5_Soluble_starch -.-> CHEBI_28017
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010168 --> ing6_Yeast_extract__BD_Difco_
ing7_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_010168 --> ing7_Proteose_peptone_No__3__BD_Difco_
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010168 --> ing8_Casamino_acids__BD_Difco_
ing9_Artificial_seawater__see_below_["Artificial seawater (see below)"]
CultureMech_010168 --> ing9_Artificial_seawater__see_below_
ing10_NaCl["NaCl"]
CultureMech_010168 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_010168 --> ing11_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_2H2O -.-> CHEBI_86158
ing12_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_010168 --> ing12_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing12_MgCl2_6H2O -.-> CHEBI_86345
ing13_H3BO3["H3BO3"]
CultureMech_010168 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_KCl["KCl"]
CultureMech_010168 --> ing14_KCl
CHEBI_32588["CHEBI:32588"]
ing14_KCl -.-> CHEBI_32588
ing15_NaHCO3["NaHCO3"]
CultureMech_010168 --> ing15_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing15_NaHCO3 -.-> CHEBI_32139
ing16_Na2SO4["Na2SO4"]
CultureMech_010168 --> ing16_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing16_Na2SO4 -.-> CHEBI_32149
ing17_SrCl2_6H2O["SrCl2·6H2O"]
CultureMech_010168 --> ing17_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing17_SrCl2_6H2O -.-> CHEBI_36385
ing18_NaF["NaF"]
CultureMech_010168 --> ing18_NaF
CHEBI_28741["CHEBI:28741"]
ing18_NaF -.-> CHEBI_28741
ing19_KBr["KBr"]
CultureMech_010168 --> ing19_KBr
CHEBI_32030["CHEBI:32030"]
ing19_KBr -.-> CHEBI_32030
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
251.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 250.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.025 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.15 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.15 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.25 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 | Soluble starch | 0.25 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 7 | Yeast extract (BD-Difco) | 0.25 G_PER_L | Properties: Undefined component | |
| 8 | Proteose peptone No. 3 (BD-Difco) | 0.25 G_PER_L | Properties: Undefined component | |
| 9 | Casamino acids (BD-Difco) | 0.25 G_PER_L | Properties: Undefined component | |
| 10 | Artificial seawater (see below) | 750 G_PER_L | ||
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
23.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.9 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.026 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
KCl
Cl.K 74.551 g/mol
|
0.66 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.19 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.9 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
SrCl2·6H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.024 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NaF
F.Na 41.988 g/mol
|
0.003 G_PER_L |
CHEBI:28741
sodium fluoride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
KBr
Br.K 119.002 g/mol
|
0.096 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M761_1_2_R2A_Medium_With_75_Artificial_Seawater (CultureMech:010168)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.660047Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M761 |
| 2026-01-28T19:48:23.219244Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:08.392679Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.806373Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010168 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.739405+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/2 R2A Medium With 75% Artificial Seawater" to "1_2_r2a_medium_with_75_artificial_seawater" for consistent matching |
| 2026-06-05T07:40:44.172978+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:32.824638+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.547115+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.029403+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5b6dd332029aea048e5dd9a14edeebad240a08efb3e1ffd9076bfd34b9d75f1c |