modified_nautilia_lithotrophica_medium

CultureMech:010184 bacterial COMPLEX LIQUID
Original name: Modified Nautilia Lithotrophica Medium

Overview

External ontology
TOGO:M776 — Modified Nautilia Lithotrophica Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M776 Original source: JCM - JCM_M751 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=751

Composition

flowchart LR
    CultureMech_010184["modified_nautilia_lithotrophica_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010184 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_010184 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010184 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_010184 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Resazurin["Resazurin"]
    CultureMech_010184 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010184 --> ing5_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_6H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_010184 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaNO3["NaNO3"]
    CultureMech_010184 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_010184 --> ing8_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing8_Sulfur__powder_ -.-> CHEBI_33403
    ing9_HEPES["HEPES"]
    CultureMech_010184 --> ing9_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing9_HEPES -.-> CHEBI_46756
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010184 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010184 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
900 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component
2 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.33 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
4 NH4Cl
Cl.H4N 53.492 g/mol
0.33 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
5 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
6 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.33 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
7 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
9 Sulfur (powder) 12 G_PER_L CHEBI:33403
elemental sulfur
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 HEPES 2.38 G_PER_L CHEBI:46756
HEPES
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

5% NaHCO3 solution*

Dose: 50 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

3% Na2S・9H2O solution

Dose: 20 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

15% Sodium formate solution

Dose: 20 G_PER_L

Properties: Defined component, Organic compound, Solution

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T06:35:55.692090Z togo-import Imported from TOGO Medium Source: JCM, ID: M776
2026-01-28T19:48:39.359759Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:05:06.157759+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T06:20:11.943947Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010184
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:30.879824+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Nautilia Lithotrophica Medium" to "modified_nautilia_lithotrophica_medium" for consistent matching
2026-05-16T09:03:20.777875+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:44.342777+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.607200+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:19.194058+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 915ba9eb8fb1cb322fa86543fb46eae761064edfeb6a1f7068e44736b1f14422