Overview
- External ontology
- TOGO:M779 — MJ Medium For Defferibacter SP. Strain Nejineji
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M779 Original source: JCM - JCM_M754 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=754
Composition
flowchart LR
CultureMech_010187["mj_medium_for_defferibacter_sp_strain_nejineji"]
ing0_Distilled_water["Distilled water"]
CultureMech_010187 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010187 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010187 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_010187 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2HPO4["K2HPO4"]
CultureMech_010187 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010187 --> ing5_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_2H2O -.-> CHEBI_75213
ing6_Resazurin["Resazurin"]
CultureMech_010187 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010187 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_H3BO3["H3BO3"]
CultureMech_010187 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_KCl["KCl"]
CultureMech_010187 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010187 --> ing10_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_4H2O -.-> CHEBI_86368
ing11_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010187 --> ing11_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing11_CoCl2_6H2O -.-> CHEBI_35696
ing12_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010187 --> ing12_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_6H2O -.-> CHEBI_34887
ing13_ZnCl2["ZnCl2"]
CultureMech_010187 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_010187 --> ing14_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing14_Na2SeO3_5H2O -.-> CHEBI_131361
ing15_Na2WO2_2H2O["Na2WO2・2H2O"]
CultureMech_010187 --> ing15_Na2WO2_2H2O
ing16_Sulfur["Sulfur"]
CultureMech_010187 --> ing16_Sulfur
CHEBI_26833["CHEBI:26833"]
ing16_Sulfur -.-> CHEBI_26833
ing17_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_010187 --> ing17_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing17_FeCl2_4H2O -.-> CHEBI_86249
ing18_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010187 --> ing18_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing18_Carbon_dioxide_gas -.-> CHEBI_16526
ing19_Nitrogen_gas["Nitrogen gas"]
CultureMech_010187 --> ing19_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing19_Nitrogen_gas -.-> CHEBI_17997
ing20_Hydrogen_gas["Hydrogen gas"]
CultureMech_010187 --> ing20_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing20_Hydrogen_gas -.-> CHEBI_18276
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.4 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
6.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
4 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.5 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
4 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 | Na2WO2・2H2O | 0.1 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 17 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.25 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 20 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 21 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
8% NaHCO3 solution
Dose: 12.5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Na2S・9H2O solution
Dose: variable VARIABLE
Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M779_MJ_Medium_For_Defferibacter_SP._Strain_Nejineji (CultureMech:010187)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.697459Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M779 |
| 2026-01-28T19:48:10.926791Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Na2S・9H2O solution; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:17.881175+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:45.525378Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:11.969291Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010187 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.905437+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ Medium For Defferibacter SP. Strain Nejineji" to "mj_medium_for_defferibacter_sp_strain_nejineji" for consistent matching |
| 2026-05-16T09:03:20.807639+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.373423+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.617329+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.223482+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e55d7c76443ee202d283fabf81c111f90ab92b619b46003d58954a86e5bcbe5f |