Overview
- External ontology
- TOGO:M780 — MMJ-S304 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M780 Original source: JCM - JCM_M755 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=755
Composition
flowchart LR
CultureMech_010189["mmj_s304_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010189 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010189 --> ing1_Yeast_extract
ing2_Resazurin["Resazurin"]
CultureMech_010189 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_NaOH["NaOH"]
CultureMech_010189 --> ing3_NaOH
CHEBI_32145["CHEBI:32145"]
ing3_NaOH -.-> CHEBI_32145
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010189 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_Nitrogen_gas["Nitrogen gas"]
CultureMech_010189 --> ing5_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing5_Nitrogen_gas -.-> CHEBI_17997
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Defined component, Organic compound |
| 4 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 6 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Modified MJ synthetic seawater (see Medium [M493])
Dose: 1 G_PER_L
Cross-reference to Medium M493
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
8% NaHCO3 solution*
Dose: 12.5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
5% Na2S・9H2O solution
Dose: 10 G_PER_L
Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M780_MMJ-S304_Medium (CultureMech:010189)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.701367Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M780 |
| 2026-01-28T19:48:14.700591Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:24.435276+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:08:52.806743Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:11.981937Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010189 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:30.918512+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ-S304 Medium" to "mmj_s304_medium" for consistent matching |
| 2026-05-16T09:03:20.820173+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.388819+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:19.241378+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 95e8d60247357bbee541007e3636504e19b7df3d8c174dd263a7beaaf3335e81 |