Overview
- External ontology
- TOGO:M798 — Betaine+bicarbonate Buffered Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M798 Original source: JCM - JCM_M770 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=770
Composition
flowchart LR
CultureMech_010208["betaine_bicarbonate_buffered_medium"]
ing0_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010208 --> ing0_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing0_Carbon_dioxide_gas -.-> CHEBI_16526
ing1_Nitrogen_gas["Nitrogen gas"]
CultureMech_010208 --> ing1_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing1_Nitrogen_gas -.-> CHEBI_17997
ing2_Distilled_water["Distilled water"]
CultureMech_010208 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
ing3_Yeast_extract["Yeast extract"]
CultureMech_010208 --> ing3_Yeast_extract
ing4_Resazurin["Resazurin"]
CultureMech_010208 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_010208 --> ing5_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing5_Na2SeO3_5H2O -.-> CHEBI_131361
ing6_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_010208 --> ing6_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing6_Na2WO4_2H2O -.-> CHEBI_63939
ing7_Na2S_9H2O["Na2S・9H2O"]
CultureMech_010208 --> ing7_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_9H2O -.-> CHEBI_76209
ing8_NaHCO3["NaHCO3"]
CultureMech_010208 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_010208 --> ing9_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing10_KH2PO4["KH2PO4"]
CultureMech_010208 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_Na2HPO4["Na2HPO4"]
CultureMech_010208 --> ing11_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing11_Na2HPO4 -.-> CHEBI_34683
ing12_32__HCl["32% HCl"]
CultureMech_010208 --> ing12_32__HCl
ing13_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010208 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_010208 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010208 --> ing15_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_4H2O -.-> CHEBI_86368
ing16_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010208 --> ing16_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_6H2O -.-> CHEBI_35696
ing17_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_010208 --> ing17_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_2H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_010208 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_AlCl3["AlCl3"]
CultureMech_010208 --> ing19_AlCl3
CHEBI_30114["CHEBI:30114"]
ing19_AlCl3 -.-> CHEBI_30114
ing20_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_010208 --> ing20_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing20_FeCl2_4H2O -.-> CHEBI_86249
ing21_NiCl2_H2O["NiCl2・H2O"]
CultureMech_010208 --> ing21_NiCl2_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_H2O -.-> CHEBI_34887
ing22_EDTA["EDTA"]
CultureMech_010208 --> ing22_EDTA
CHEBI_4735["CHEBI:4735"]
ing22_EDTA -.-> CHEBI_4735
ing23_NaCl["NaCl"]
CultureMech_010208 --> ing23_NaCl
CHEBI_26710["CHEBI:26710"]
ing23_NaCl -.-> CHEBI_26710
ing24_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010208 --> ing24_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing24_CaCl2_2H2O -.-> CHEBI_86158
ing25_NH4Cl["NH4Cl"]
CultureMech_010208 --> ing25_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing25_NH4Cl -.-> CHEBI_31206
ing26_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010208 --> ing26_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing26_MgCl2_6H2O -.-> CHEBI_86345
ing27_Biotin["Biotin"]
CultureMech_010208 --> ing27_Biotin
CHEBI_15956["CHEBI:15956"]
ing27_Biotin -.-> CHEBI_15956
ing28_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_010208 --> ing28_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p__Aminobenzoic_acid -.-> CHEBI_30753
ing29_Thiamine_HCl["Thiamine・HCl"]
CultureMech_010208 --> ing29_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing29_Thiamine_HCl -.-> CHEBI_49105
CultureMech_010208 --> more["...9 more"]
Ingredients (39)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 2 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 3 |
Distilled water
H2O 18.015 g/mol
|
1078.5 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 900.0, 25.5, 50.0, 100.0, 1.0, 1.0, 1.0] |
| 4 | Yeast extract | 0.2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.3 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.25 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Organic compound, Simple component |
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.43 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 13 | 32% HCl | 1 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Solution, Defined component | |
| 14 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.05 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.05 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.03 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.05 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
AlCl3
AlCl3 133.341 g/mol
|
0.05 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
2 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
NiCl2・H2O
Cl2Ni 129.599 g/mol
|
0.05 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Simple component, Organic compound |
| 24 |
NaCl
Cl.Na 58.443 g/mol
|
24 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
8.8 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
NH4Cl
Cl.H4N 53.492 g/mol
|
24 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.1 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.1 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 37 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 38 |
Pyridoxamine
C8H12N2O2 168.196 g/mol
|
0.25 G_PER_L |
CHEBI:16410
pyridoxamine
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 39 | DL--Calcium pantothenate | 0.05 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Solution A
Dose: 915 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 50 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Solution C
Dose: 50 G_PER_L
See mediadive_5312_Solution_C.yaml for composition
Phosphate solution (see below)
Dose: 15 G_PER_L
1.0 M Betaine solution
Dose: 10 G_PER_L
Role: Growth factor; Properties: Defined component, Organic compound, Solution
Trace metal solution (see below)
Dose: 1 G_PER_L
Vitamin solution (see below)
Dose: 1 G_PER_L
Mineral salt solution (see below)
Dose: 12.5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M798_Betaine_bicarbonate_Buffered_Medium (CultureMech:010208)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.730313Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M798 |
| 2026-01-28T19:48:37.343674Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:05:02.289176+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 8 solution(s) from ingredients |
| 2026-03-13T03:09:34.169531Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 12 duplicate ingredient(s) |
| 2026-03-13T06:20:12.117370Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010208 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.058052+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Betaine+bicarbonate Buffered Medium" to "betaine_bicarbonate_buffered_medium" for consistent matching |
| 2026-05-16T09:03:20.954641+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.533926+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:34.988053+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:51.666845+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.382769+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bed093bcc6ce08284705312d3f767b63ecab563259769f142b7c5e6db349b627 |