Overview
- External ontology
- TOGO:M801 — Saltwater Medium With Hydrogen
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M801 Original source: JCM - JCM_M772 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=772
Composition
flowchart LR
CultureMech_010213["saltwater_medium_with_hydrogen"]
ing0_Distilled_water["Distilled water"]
CultureMech_010213 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010213 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010213 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_010213 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_010213 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_010213 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010213 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_010213 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Na2SO4["Na2SO4"]
CultureMech_010213 --> ing8_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing8_Na2SO4 -.-> CHEBI_32149
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010213 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_Hydrogen_gas["Hydrogen gas"]
CultureMech_010213 --> ing10_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing10_Hydrogen_gas -.-> CHEBI_18276
ing11_5__Na2S_9H2O["5% Na2S・9H2O"]
CultureMech_010213 --> ing11_5__Na2S_9H2O
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
960 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 9 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 11 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 12 | 5% Na2S・9H2O | 8 G_PER_L | Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound |
Solutions
8% NaHCO3 solution*
Dose: 30 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Trace element solution (see Medium [M439])
Dose: 1 G_PER_L
Cross-reference to Medium M439
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
Vitamin solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Thiamine solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Vitamin B12 solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Riboflavin solution (see Medium [M463])
Dose: 1 G_PER_L
Cross-reference to Medium M463
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M801_Saltwater_Medium_With_Hydrogen (CultureMech:010213)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.742087Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M801 |
| 2026-01-28T19:48:10.688734Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:17.524623+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:08:45.101861Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:12.154744Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010213 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.096315+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Saltwater Medium With Hydrogen" to "saltwater_medium_with_hydrogen" for consistent matching |
| 2026-05-16T09:03:20.993229+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.577627+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.679772+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.435971+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 318a9a49c086a8c8e1a2d624ceb29544f73cc5745f5462537756ce173f55794f |