rumen_fluid_medium

CultureMech:010214 bacterial COMPLEX LIQUID
Original name: Rumen Fluid Medium

Overview

External ontology
TOGO:M802 — Rumen Fluid Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M802 Original source: JCM - JCM_M773 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=773

Composition

flowchart LR
    CultureMech_010214["rumen_fluid_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010214 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010214 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_010214 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010214 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_010214 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_K2HPO4["K2HPO4"]
    CultureMech_010214 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_010214 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_010214 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_010214 --> ing8_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_7H2O -.-> CHEBI_75836
    ing9_NaHCO3["NaHCO3"]
    CultureMech_010214 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010214 --> ing10__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing10__NH4_2SO4 -.-> CHEBI_62946
    ing11_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_010214 --> ing11_Yeast_extract__BD_Difco_
    ing12_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_010214 --> ing12_Trypticase_peptone__BD_BBL_
    ing13_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010214 --> ing13_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing13_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010214 --> ing14_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing14_Carbon_dioxide_gas -.-> CHEBI_16526
    ing15_N2["N2"]
    CultureMech_010214 --> ing15_N2
    CHEBI_17997["CHEBI:17997"]
    ing15_N2 -.-> CHEBI_17997
    ing16_Hydrogen_gas["Hydrogen gas"]
    CultureMech_010214 --> ing16_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing16_Hydrogen_gas -.-> CHEBI_18276

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
660 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.13 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 NaCl
Cl.Na 58.443 g/mol
0.6 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
8 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
7 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Inorganic compound, Simple component, Defined component
9 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
11 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.3 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
12 Yeast extract (BD-Difco) 1 G_PER_L Properties: Undefined component
13 Trypticase peptone (BD-BBL) 1 G_PER_L Properties: Undefined component
14 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
15 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
16 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
17 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス

Solutions

Rumen fluid, clarified (see Medium [M258])

Dose: 300 G_PER_L

Cross-reference to Medium M258

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.744307Z togo-import Imported from TOGO Medium Source: JCM, ID: M802
2026-01-28T19:48:08.669010Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:13.982910+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:20:12.164899Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010214
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.106401+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Rumen Fluid Medium" to "rumen_fluid_medium" for consistent matching
2026-05-16T09:03:21.005052+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:44.590023+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:33.124485+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.684521+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:19.450770+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 21f48d108d4f9a61e6560ec74cd6f5f0ef7502bc522ebdb9ea0cf4c2c8a872cd