Overview
- External ontology
- TOGO:M805 — MH1 Medium For Saliarchaeum Rubrodurum
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M805 Original source: JCM - JCM_M776 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=776
Composition
flowchart LR
CultureMech_010217["mh1_medium_for_saliarchaeum_rubrodurum"]
ing0_Distilled_water["Distilled water"]
CultureMech_010217 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010217 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KH2PO4["KH2PO4"]
CultureMech_010217 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_010217 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010217 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_K2SO4["K2SO4"]
CultureMech_010217 --> ing5_K2SO4
CHEBI_32036["CHEBI:32036"]
ing5_K2SO4 -.-> CHEBI_32036
ing6_L__Glutamic_acid["L--Glutamic acid"]
CultureMech_010217 --> ing6_L__Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing6_L__Glutamic_acid -.-> CHEBI_16015
ing7_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
CultureMech_010217 --> ing7_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing7_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010217 --> ing8_Yeast_extract__BD_Difco_
ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010217 --> ing9_Casamino_acids__BD_Difco_
ing10_KOH["KOH"]
CultureMech_010217 --> ing10_KOH
CHEBI_32035["CHEBI:32035"]
ing10_KOH -.-> CHEBI_32035
ing11_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010217 --> ing11_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_2H2O -.-> CHEBI_86158
ing12_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010217 --> ing12_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_2H2O -.-> CHEBI_75213
ing13_H3BO4["H3BO4"]
CultureMech_010217 --> ing13_H3BO4
ing14_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010217 --> ing14_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_4H2O -.-> CHEBI_86368
ing15_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010217 --> ing15_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2_6H2O -.-> CHEBI_35696
ing16_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010217 --> ing16_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_6H2O -.-> CHEBI_34887
ing17_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_010217 --> ing17_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_2H2O -.-> CHEBI_86318
ing18_ZnCl2["ZnCl2"]
CultureMech_010217 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_010217 --> ing19_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_2H2O -.-> CHEBI_63939
ing20_AlCl3["AlCl3"]
CultureMech_010217 --> ing20_AlCl3
CHEBI_30114["CHEBI:30114"]
ing20_AlCl3 -.-> CHEBI_30114
ing21_BaCl2_2H2O["BaCl2・2H2O"]
CultureMech_010217 --> ing21_BaCl2_2H2O
CHEBI_86153["CHEBI:86153"]
ing21_BaCl2_2H2O -.-> CHEBI_86153
ing22_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_010217 --> ing22_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing22_Na2S2O3_5H2O -.-> CHEBI_32150
ing23_HCl["HCl"]
CultureMech_010217 --> ing23_HCl
CHEBI_17883["CHEBI:17883"]
ing23_HCl -.-> CHEBI_17883
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
L--Glutamic acid
C5H9NO4 147.13 g/mol
|
2 G_PER_L |
CHEBI:16015
L-glutamic acid
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 8 |
Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
|
2 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 9 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 10 | Casamino acids (BD-Difco) | 4 G_PER_L | Properties: Undefined component | |
| 11 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 12 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | H3BO4 | 0.02 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 15 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.3 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.02 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
AlCl3
AlCl3 133.341 g/mol
|
0.04 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
BaCl2・2H2O
Ba.2Cl.2H2O 244.264 g/mol
|
0.1 G_PER_L |
CHEBI:86153
barium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Inorganic compound, Simple component, Defined component |
| 24 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Solutions
0.2% FeSO4・6H2O solution
Dose: 2 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
Trace metal solution (see below)
Dose: 2 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M805_MH1_Medium_For_Saliarchaeum_Rubrodurum (CultureMech:010217)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.748775Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M805 |
| 2026-01-28T19:48:42.890386Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH; Added default concentration for ingredient: HCl |
| 2026-03-09T08:05:12.304213+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:44.178127Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:12.186679Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010217 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.127577+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MH1 Medium For Saliarchaeum Rubrodurum" to "mh1_medium_for_saliarchaeum_rubrodurum" for consistent matching |
| 2026-05-16T09:03:21.029823+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.612899+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.693294+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.478761+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2eb8bad1b17c0a87db75a3a1bc391dc5c24c208430a779b85e6c7712ea0185aa |