Overview
- External ontology
- TOGO:M80 — SM Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M80 Original source: JCM - JCM_M89 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=89
Composition
flowchart LR
CultureMech_010222["sm_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010222 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010222 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_010222 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_010222 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Na2HPO4["Na2HPO4"]
CultureMech_010222 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010222 --> ing5_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_7H2O -.-> CHEBI_75836
ing6_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010222 --> ing6_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_4H2O -.-> CHEBI_86368
ing7_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010222 --> ing7_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing7_CoCl2_6H2O -.-> CHEBI_35696
ing8_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010222 --> ing8_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_7H2O -.-> CHEBI_32312
ing9_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010222 --> ing9_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing9_CuSO4_5H2O -.-> CHEBI_31440
ing10_CaCl2["CaCl2"]
CultureMech_010222 --> ing10_CaCl2
CHEBI_3312["CHEBI:3312"]
ing10_CaCl2 -.-> CHEBI_3312
ing11__NH4_6Mo7O24_4H2O["(NH4)6Mo7O24・4H2O"]
CultureMech_010222 --> ing11__NH4_6Mo7O24_4H2O
CHEBI_91249["CHEBI:91249"]
ing11__NH4_6Mo7O24_4H2O -.-> CHEBI_91249
ing12_EDTA["EDTA"]
CultureMech_010222 --> ing12_EDTA
CHEBI_4735["CHEBI:4735"]
ing12_EDTA -.-> CHEBI_4735
ing13_KOH["KOH"]
CultureMech_010222 --> ing13_KOH
CHEBI_32035["CHEBI:32035"]
ing13_KOH -.-> CHEBI_32035
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
901.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
4.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
4.99 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
5.06 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
1.61 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.57 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 11 |
CaCl2
Ca.2Cl 110.984 g/mol
|
5.54 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 12 |
(NH4)6Mo7O24・4H2O
2H4N.MoO4 196.014 g/mol
|
1.1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 13 |
EDTA
C10H16N2O8 292.244 g/mol
|
50 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Organic compound, Simple component |
| 14 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
10% Na2S2O3 solution
Dose: 100 G_PER_L
Properties: Defined component, Inorganic compound, Solution
Trace metal solution (see below)
Dose: 5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M80_SM_Medium (CultureMech:010222)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.738462Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M80 |
| 2026-01-28T19:48:41.831502Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-09T08:05:10.525669+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:42.302990Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:12.231117Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010222 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.171710+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SM Medium" to "sm_medium" for consistent matching |
| 2026-05-16T09:03:21.080669+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:44.665988+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.181967+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.711318+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.528351+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3e6b4911bac7da83e999d980efd396053f0b1c1f8d2448fc5f9688722659f56b |