methanobacterium_medium_iii

CultureMech:010240 archaea COMPLEX LIQUID
Original name: Methanobacterium Medium (III)

Overview

External ontology
TOGO:M827 — Methanobacterium Medium (III)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M827 Original source: JCM - JCM_M794 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=794

Composition

flowchart LR
    CultureMech_010240["methanobacterium_medium_iii"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010240 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010240 --> ing1_Yeast_extract
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010240 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_010240 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_010240 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_010240 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010240 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_NaHCO3["NaHCO3"]
    CultureMech_010240 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Sodium_acetate["Sodium acetate"]
    CultureMech_010240 --> ing8_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Sodium_acetate -.-> CHEBI_32954
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010240 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_N2["N2"]
    CultureMech_010240 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997
    ing11_Hydrogen_gas["Hydrogen gas"]
    CultureMech_010240 --> ing11_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing11_Hydrogen_gas -.-> CHEBI_18276
    ing12_5__L__Cysteine_HCl_H2O["5% L--Cysteine・HCl・H2O"]
    CultureMech_010240 --> ing12_5__L__Cysteine_HCl_H2O

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 0.2 G_PER_L Role: Nutrient source; Properties: Undefined component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.147 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.136 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
5 NH4Cl
Cl.H4N 53.492 g/mol
0.54 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
6 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 NaHCO3
CHO3.Na 84.006 g/mol
2.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
9 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.8 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Defined component, Organic compound, Simple component
10 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
12 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
13 5% L--Cysteine・HCl・H2O 10 G_PER_L Properties: Defined component, Organic compound, Solution

Solutions

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

5% Na2S・9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.790922Z togo-import Imported from TOGO Medium Source: JCM, ID: M827
2026-01-28T19:48:08.333631Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:04:13.431266+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:08:40.408515Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:20:12.369874Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010240
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.313473+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanobacterium Medium (III)" to "methanobacterium_medium_iii" for consistent matching
2026-05-16T09:03:21.224896+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:44.830348+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.758788+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:27.802432+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 5562794300d9ac1396ad587106016a4a5b64fbcbd2275aa68538cba0e9071cfd