Overview
- External ontology
- TOGO:M82 — S6 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M82 Original source: JCM - JCM_M90-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=90
Composition
flowchart LR
CultureMech_010243["s6_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010243 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010243 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010243 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_010243 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_010243 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_010243 --> ing5_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_6H2O -.-> CHEBI_86254
ing6_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010243 --> ing6_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing6_MnSO4_xH2O -.-> CHEBI_86360
ing7_Na2HPO4["Na2HPO4"]
CultureMech_010243 --> ing7_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing7_Na2HPO4 -.-> CHEBI_34683
ing8_agar["agar"]
CultureMech_010243 --> ing8_agar
CHEBI_2509["CHEBI:2509"]
ing8_agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
40 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.8 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
30 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
30 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
1.2 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 9 | agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
Solutions
10% Na2S2O3 solution
Dose: 100 G_PER_L
Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M82_S6_Medium (CultureMech:010243)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.778079Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M82 |
| 2026-01-28T19:48:42.203238Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:11.131049+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:12.384066Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010243 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.327116+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "S6 Medium" to "s6_medium" for consistent matching |
| 2026-05-16T09:03:21.237675+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:44.845819+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.317719+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.763114+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:19.653731+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6a863f121b870b92123982faaa222ca174bdb1443c75d1e0a645cfd13f781726 |