Overview
- External ontology
- TOGO:M845 — Thermococcales Rich Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M845 Original source: JCM - JCM_M811 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=811
Composition
flowchart LR
CultureMech_010260["thermococcales_rich_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010260 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_010260 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_010260 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010260 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010260 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_010260 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_010260 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010260 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_Na2S_9H2O["Na2S・9H2O"]
CultureMech_010260 --> ing8_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_9H2O -.-> CHEBI_76209
ing9_KCl["KCl"]
CultureMech_010260 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_010260 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_010260 --> ing11_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing11_FeCl3_6H2O -.-> CHEBI_86254
ing12_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_010260 --> ing12_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing12_Na2WO4_2H2O -.-> CHEBI_63939
ing13_Sulfur__powder_["Sulfur (powder)"]
CultureMech_010260 --> ing13_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing13_Sulfur__powder_ -.-> CHEBI_33403
ing14_NaBr["NaBr"]
CultureMech_010260 --> ing14_NaBr
CHEBI_63004["CHEBI:63004"]
ing14_NaBr -.-> CHEBI_63004
ing15_SrCl2_6H2O["SrCl2・6H2O"]
CultureMech_010260 --> ing15_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing15_SrCl2_6H2O -.-> CHEBI_36385
ing16_PIPES["PIPES"]
CultureMech_010260 --> ing16_PIPES
CHEBI_39033["CHEBI:39033"]
ing16_PIPES -.-> CHEBI_39033
ing17_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010260 --> ing17_Tryptone__BD_Difco_
ing18_N2["N2"]
CultureMech_010260 --> ing18_N2
CHEBI_17997["CHEBI:17997"]
ing18_N2 -.-> CHEBI_17997
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
23 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.05 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.7 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
6.8 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
3.3 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
SrCl2・6H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.01 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 | PIPES | 3.3 G_PER_L |
CHEBI:39033
PIPES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 18 | Tryptone (BD-Difco) | 4 G_PER_L | Properties: Undefined component | |
| 19 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M845_Thermococcales_Rich_Medium (CultureMech:010260)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.817417Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M845 |
| 2026-01-28T19:48:09.892011Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-13T06:20:12.488093Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010260 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.432182+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Thermococcales Rich Medium" to "thermococcales_rich_medium" for consistent matching |
| 2026-05-16T09:03:21.345354+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:44.970143+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.795517+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:27.826957+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a2de9d584bb36f13173152bcbd173d778bec68733faea91d2880b5beacc38362 |