Overview
- External ontology
- TOGO:M875 — R2A Broth
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M875 Original source: JCM - JCM_M839 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=839
Composition
flowchart LR
CultureMech_010293["r2a_broth"]
ing0_Distilled_water["Distilled water"]
CultureMech_010293 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010293 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_010293 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_010293 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_Glucose["Glucose"]
CultureMech_010293 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Soluble_starch["Soluble starch"]
CultureMech_010293 --> ing5_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing5_Soluble_starch -.-> CHEBI_28017
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010293 --> ing6_Yeast_extract__BD_Difco_
ing7_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_010293 --> ing7_Proteose_peptone_No__3__BD_Difco_
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_010293 --> ing8_Casamino_acids__BD_Difco_
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 6 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 7 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 8 | Proteose peptone No. 3 (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 9 | Casamino acids (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M875_R2A_Broth (CultureMech:010293)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.880283Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M875 |
| 2026-01-28T19:48:10.088698Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:20:12.758178Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010293 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.703371+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A Broth" to "r2a_broth" for consistent matching |
| 2026-06-05T07:40:45.266125+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.644001+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:51.907435+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.102964+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3242a67dd9b68d1f42f981a7e2dc50882f2f98e11c0d436eb0722f7b7f396246 |