lactate_bicarbonate_buffered_medium

CultureMech:010311 bacterial COMPLEX LIQUID
Original name: Lactate+bicarbonate Buffered Medium

Overview

External ontology
TOGO:M893 — Lactate+bicarbonate Buffered Medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'glucose_bicarbonate_buffered_medium', 'source': 'TOGO', 'source_id': 'TOGO:M1377', 'original_category': 'bacterial'}
Notes
Source: https://togomedium.org/medium/M893 Original source: JCM - JCM_M857 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=857

Composition

flowchart LR
    CultureMech_010311["lactate_bicarbonate_buffered_medium"]
    ing0_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010311 --> ing0_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing0_Carbon_dioxide_gas -.-> CHEBI_16526
    ing1_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010311 --> ing1_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing1_Nitrogen_gas -.-> CHEBI_17997
    ing2_Distilled_water["Distilled water"]
    CultureMech_010311 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_010311 --> ing3_Yeast_extract
    ing4_Resazurin["Resazurin"]
    CultureMech_010311 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Na2SeO3_5H2O["Na2SeO3・5H2O"]
    CultureMech_010311 --> ing5_Na2SeO3_5H2O
    CHEBI_131361["CHEBI:131361"]
    ing5_Na2SeO3_5H2O -.-> CHEBI_131361
    ing6_Na2WO4_2H2O["Na2WO4・2H2O"]
    CultureMech_010311 --> ing6_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing6_Na2WO4_2H2O -.-> CHEBI_63939
    ing7_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_010311 --> ing7_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_9H2O -.-> CHEBI_76209
    ing8_NaHCO3["NaHCO3"]
    CultureMech_010311 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010311 --> ing9_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
2 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
3 Distilled water
H2O 18.015 g/mol
975.5 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 900.0, 25.5, 50.0]
4 Yeast extract 0.2 G_PER_L Role: Nutrient source; Properties: Undefined component
5 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
6 Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
0.3 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
7 Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
0.3 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
0.25 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Defined component, Simple component, Inorganic compound
9 NaHCO3
CHO3.Na 84.006 g/mol
4 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
10 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component

Solutions

Solution A

Dose: 900 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 50 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Solution C

Dose: 50 G_PER_L

See mediadive_5312_Solution_C.yaml for composition

Phosphate solution (see Medium [M798])

Dose: 15 G_PER_L

Cross-reference to Medium M798

lactate solution

Dose: 10 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

Trace metal solution (see Medium [M798])

Dose: 1 G_PER_L

Cross-reference to Medium M798

Vitamin solution (see Medium [M798])

Dose: 1 G_PER_L

Cross-reference to Medium M798

Mineral salt solution (see Medium [M798])

Dose: 12.5 G_PER_L

Cross-reference to Medium M798

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:55.913920Z togo-import Imported from TOGO Medium Source: JCM, ID: M893
2026-01-28T19:48:09.833426Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:15.980329+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 8 solution(s) from ingredients
2026-03-13T03:08:43.334683Z data-quality-cleanup-v1.0 Data quality fixes Merged 8 duplicate ingredient(s)
2026-03-13T06:20:12.909650Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010311
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.855863+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Lactate+bicarbonate Buffered Medium" to "lactate_bicarbonate_buffered_medium" for consistent matching
2026-05-16T09:03:21.803485+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.443599+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:08.456421+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: TOGO_M1377_Glucose_bicarbonate-Buffered_Medium, TOGO_M893_Lactate_bicarbonate_Buffered_Medium
2026-08-06T03:05:08.456463+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 660330623f57b013e3dc19bc6dd8cafa1bdb260c125ec96f4173587dfa38afde