Overview
- External ontology
- TOGO:M899 — Steroidobacter Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M899 Original source: JCM - JCM_M862 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=862
Composition
flowchart LR
CultureMech_010316["steroidobacter_medium"]
ing0_Sol__1["Sol. 1"]
CultureMech_010316 --> ing0_Sol__1
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010316 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_Nitrogen_gas["Nitrogen gas"]
CultureMech_010316 --> ing2_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing2_Nitrogen_gas -.-> CHEBI_17997
ing3_Distilled_water["Distilled water"]
CultureMech_010316 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_NaCl["NaCl"]
CultureMech_010316 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010316 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_KH2PO4["KH2PO4"]
CultureMech_010316 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NH4Cl["NH4Cl"]
CultureMech_010316 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010316 --> ing8_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_6H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_010316 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NaNO3["NaNO3"]
CultureMech_010316 --> ing10_NaNO3
CHEBI_63005["CHEBI:63005"]
ing10_NaNO3 -.-> CHEBI_63005
ing11_Na2SO4["Na2SO4"]
CultureMech_010316 --> ing11_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing11_Na2SO4 -.-> CHEBI_32149
ing12_testosterone["testosterone"]
CultureMech_010316 --> ing12_testosterone
ing13_acetone["acetone"]
CultureMech_010316 --> ing13_acetone
ing14_CO2["CO2"]
CultureMech_010316 --> ing14_CO2
ing14_CO2 -.-> CHEBI_16526
ing15_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_010316 --> ing15_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing15_Na2SeO3_5H2O -.-> CHEBI_131361
ing16_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_010316 --> ing16_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing16_Na2WO4_2H2O -.-> CHEBI_63939
ing17_NaOH["NaOH"]
CultureMech_010316 --> ing17_NaOH
CHEBI_32145["CHEBI:32145"]
ing17_NaOH -.-> CHEBI_32145
ing18_Biotin["Biotin"]
CultureMech_010316 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_010316 --> ing19_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p__Aminobenzoic_acid -.-> CHEBI_30753
ing20_Folic_acid["Folic acid"]
CultureMech_010316 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Vitamin_B12["Vitamin B12"]
CultureMech_010316 --> ing21_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing21_Vitamin_B12 -.-> CHEBI_176843
ing22_Riboflavin["Riboflavin"]
CultureMech_010316 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_010316 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Nicotinamide["Nicotinamide"]
CultureMech_010316 --> ing24_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing24_Nicotinamide -.-> CHEBI_17154
ing25_Thiamine_HCl_2H2O["Thiamine・HCl・2H2O"]
CultureMech_010316 --> ing25_Thiamine_HCl_2H2O
ing26_Pantothenic_acid["Pantothenic acid"]
CultureMech_010316 --> ing26_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing26_Pantothenic_acid -.-> CHEBI_7916
ing27_Pyridoxamine_HCl["Pyridoxamine・HCl"]
CultureMech_010316 --> ing27_Pyridoxamine_HCl
ing28____Lipoic_acid["α--Lipoic acid"]
CultureMech_010316 --> ing28____Lipoic_acid
CHEBI_43796["CHEBI:43796"]
ing28____Lipoic_acid -.-> CHEBI_43796
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sol. 1 | 10 G_PER_L | ||
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 3 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 4 |
Distilled water
H2O 18.015 g/mol
|
4.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 4 duplicates: 1.0, 1.0, 1.0, 1.0] |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaNO3
NO3.Na 84.994 g/mol
|
0.42 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.07 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 | testosterone | 20 G_PER_L | Role: Growth factor; Properties: Defined component, Simple component | |
| 14 | acetone | 1 G_PER_L | Role: Solvating media; Properties: Organic compound, Defined component, Simple component | |
| 15 |
CO2
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 16 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
4 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
20 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 20 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
50 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
20 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 22 | Vitamin B12 | 50 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
50 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
25 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
25 G_PER_L |
CHEBI:17154
nicotinamide
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 26 | Thiamine・HCl・2H2O | 50 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 27 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
50 G_PER_L |
CHEBI:7916
pantothenic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 | Pyridoxamine・HCl | 10 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 29 |
α--Lipoic acid
C8H14O2S2 206.332 g/mol
|
50 G_PER_L |
CHEBI:43796
(S)-lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
testosterone solution
Dose: 0.1 G_PER_L
NaHCO3 solution
Dose: 0.3 G_PER_L
Trace element solution SL--10 (see Medium [M433])
Dose: 1 G_PER_L
Cross-reference to Medium M433
Selenite--tungstate solution
Dose: 1 G_PER_L
Vitamin solution
Dose: 1 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
NaHCO3 solution
Dose: 84 G_PER_L
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M899_Steroidobacter_Medium (CultureMech:010316)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.922005Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M899 |
| 2026-01-28T19:48:25.140655Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: CO2 |
| 2026-03-09T08:04:41.562057+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:11.563828Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:20:12.951671Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010316 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.897110+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Steroidobacter Medium" to "steroidobacter_medium" for consistent matching |
| 2026-05-16T09:03:21.852016+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.491856+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:51.978984+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.326378+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 044383689c8451ce40bda93a27f1f4c19070216cd2bbac0cf12d3827a9658b41 |