ml_medium

CultureMech:010320 bacterial COMPLEX LIQUID
Original name: ML Medium

Overview

External ontology
TOGO:M901 — ML Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M901 Original source: JCM - JCM_M864 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=864

Composition

flowchart LR
    CultureMech_010320["ml_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010320 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010320 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_010320 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NaHCO3["NaHCO3"]
    CultureMech_010320 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010320 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_Na2CO3["Na2CO3"]
    CultureMech_010320 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Na2B4O7_10H2O["Na2B4O7・10H2O"]
    CultureMech_010320 --> ing6_Na2B4O7_10H2O
    CHEBI_131366["CHEBI:131366"]
    ing6_Na2B4O7_10H2O -.-> CHEBI_131366

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
70 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 NaHCO3
CHO3.Na 84.006 g/mol
6.8 G_PER_L CHEBI:32139
sodium hydrogencarbonate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
6 Na2CO3
CO3.2Na 105.988 g/mol
32 G_PER_L CHEBI:29377
sodium carbonate
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7 Na2B4O7・10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
3.8 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.930046Z togo-import Imported from TOGO Medium Source: JCM, ID: M901
2026-01-28T19:48:09.039992Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:20:12.972411Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010320
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.917974+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ML Medium" to "ml_medium" for consistent matching
2026-06-05T07:40:45.515431+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.986656+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:20.350238+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint aa52b3e704ff7320221b4b5f9720948a2e95ddc76ac10e6c267d9a53f834dab7