desulfosporosinus_bsrei1_medium

CultureMech:010321 bacterial COMPLEX LIQUID
Original name: Desulfosporosinus Bsrei1 Medium

Overview

External ontology
TOGO:M902 — Desulfosporosinus Bsrei1 Medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'desulfosporosinus_acidophilus_medium', 'source': 'TOGO', 'source_id': 'TOGO:M814', 'original_category': 'bacterial'}
Notes
Source: https://togomedium.org/medium/M902 Original source: JCM - JCM_M865 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=865

Composition

flowchart LR
    CultureMech_010321["desulfosporosinus_bsrei1_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010321 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010321 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_010321 --> ing2_Yeast_extract
    ing3_KH2PO4["KH2PO4"]
    CultureMech_010321 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Resazurin["Resazurin"]
    CultureMech_010321 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_KCl["KCl"]
    CultureMech_010321 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010321 --> ing6__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing6__NH4_2SO4 -.-> CHEBI_62946
    ing7_Na2SO4["Na2SO4"]
    CultureMech_010321 --> ing7_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing7_Na2SO4 -.-> CHEBI_32149
    ing8_Ca_NO3_2_4H2O["Ca(NO3)2・4H2O"]
    CultureMech_010321 --> ing8_Ca_NO3_2_4H2O
    CHEBI_86159["CHEBI:86159"]
    ing8_Ca_NO3_2_4H2O -.-> CHEBI_86159
    ing9_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_010321 --> ing9_L__Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L__Cysteine_HCl_H2O -.-> CHEBI_91248
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010321 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010321 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
900 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 Yeast extract 0.5 G_PER_L Role: Nutrient source; Properties: Undefined component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.05 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
6 KCl
Cl.K 74.551 g/mol
0.05 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.45 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
8 Na2SO4
2Na.O4S 142.043 g/mol
3 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
9 Ca(NO3)2・4H2O
Ca.4H2O.2NO3 236.146 g/mol
0.014 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10 L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace element solution (see Medium [M439])

Dose: 1 G_PER_L

Cross-reference to Medium M439

5% NaHCO3 solution*

Dose: 60 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1 M Fructose solution

Dose: 20 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

5% Na2S・9H2O solution

Dose: 8 G_PER_L

Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:55.931256Z togo-import Imported from TOGO Medium Source: JCM, ID: M902
2026-01-28T19:48:12.981365Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:21.459640+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T06:20:12.980209Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010321
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.925488+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Desulfosporosinus Bsrei1 Medium" to "desulfosporosinus_bsrei1_medium" for consistent matching
2026-05-16T09:03:21.877634+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.524603+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:33.820719+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:51.989395+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:19.577535+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: TOGO_M814_Desulfosporosinus_Acidophilus_Medium, TOGO_M902_Desulfosporosinus_Bsrei1_Medium
2026-08-06T03:05:19.577582+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 24097ffc05831c85734106673815326c6a2f854dcec1617c1584a2e383b6cf85