desulfotomaculum_bs_medium

CultureMech:010322 bacterial COMPLEX LIQUID
Original name: Desulfotomaculum BS Medium

Overview

External ontology
TOGO:M903 — Desulfotomaculum BS Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M903 Original source: JCM - JCM_M866 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=866

Composition

flowchart LR
    CultureMech_010322["desulfotomaculum_bs_medium"]
    ing0_Sol__1["Sol. 1"]
    CultureMech_010322 --> ing0_Sol__1
    ing1_Sol__2["Sol. 2"]
    CultureMech_010322 --> ing1_Sol__2
    ing2_Distilled_water["Distilled water"]
    CultureMech_010322 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377
    ing3_NaCl["NaCl"]
    CultureMech_010322 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010322 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_010322 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_010322 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_K2HPO4["K2HPO4"]
    CultureMech_010322 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_Resazurin["Resazurin"]
    CultureMech_010322 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010322 --> ing9_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_6H2O -.-> CHEBI_86345
    ing10_KCl["KCl"]
    CultureMech_010322 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_Na2SO4["Na2SO4"]
    CultureMech_010322 --> ing11_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing11_Na2SO4 -.-> CHEBI_32149
    ing12_KOH["KOH"]
    CultureMech_010322 --> ing12_KOH
    CHEBI_32035["CHEBI:32035"]
    ing12_KOH -.-> CHEBI_32035
    ing13_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010322 --> ing13_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing13_Carbon_dioxide_gas -.-> CHEBI_16526
    ing14_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010322 --> ing14_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing14_Nitrogen_gas -.-> CHEBI_17997

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Sol. 1 9.4 G_PER_L
2 Sol. 2 0.64 G_PER_L
3 Distilled water
H2O 18.015 g/mol
940 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
4 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
5 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
7 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound
8 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
9 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Simple component, Organic compound, Defined component
10 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
11 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
12 Na2SO4
2Na.O4S 142.043 g/mol
3 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
13 KOH
HO.K 56.105 g/mol
variable VARIABLE CHEBI:32035
potassium hydroxide
 Role: Mineral source; Properties: Defined component, Solution
14 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
15 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace metal solution (see Medium [M288])

Dose: 1 G_PER_L

Cross-reference to Medium M288

8% NaHCO3 solution*

Dose: 0.25 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1 M Sodium pyruvate solution

Dose: 0.2 G_PER_L

Role: Carbon source; Properties: Defined component, Organic compound, Solution

5% Na2S・9H2O solution

Dose: 0.04 G_PER_L

Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component

1% Sodium dithionite solution* (freshly prepared)

Dose: 0.1 G_PER_L

Properties: Defined component, Inorganic compound, Solution

Trace vitamins (see Medium [M190])

Dose: 0.05 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.933380Z togo-import Imported from TOGO Medium Source: JCM, ID: M903
2026-01-28T19:48:33.222259Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: KOH; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas
2026-03-09T08:04:55.042022+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T06:20:12.990630Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010322
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:35.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:31.935840+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Desulfotomaculum BS Medium" to "desulfotomaculum_bs_medium" for consistent matching
2026-05-16T09:03:21.889333+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.536513+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:51.993798+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:20.361673+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint c95bda333874e19fb6db0db7bc06bb0080945a7aa4e8062a4e2044e4e781bd0e