Overview
- External ontology
- TOGO:M90 — Alkaline Lactose Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M90 Original source: JCM - JCM_M98 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=98
Composition
flowchart LR
CultureMech_010329["alkaline_lactose_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_010329 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010329 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_010329 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_010329 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Lactose["Lactose"]
CultureMech_010329 --> ing4_Lactose
CHEBI_36218["CHEBI:36218"]
ing4_Lactose -.-> CHEBI_36218
ing5_Agar["Agar"]
CultureMech_010329 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Polypepton__Nihon_Pharm__Co__["Polypepton (Nihon Pharm. Co.)"]
CultureMech_010329 --> ing6_Polypepton__Nihon_Pharm__Co__
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
Lactose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:36218
beta-lactose
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 | Polypepton (Nihon Pharm. Co.) | 5 G_PER_L | Properties: Undefined component |
Solutions
10% Na2CO3 solution
Dose: 100 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M90_Alkaline_Lactose_Agar (CultureMech:010329)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.928082Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M90 |
| 2026-01-28T19:48:40.961229Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:08.938695+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:13.026933Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010329 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:31.972179+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Lactose Agar" to "alkaline_lactose_agar" for consistent matching |
| 2026-05-16T09:03:21.928419+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.588137+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:33.865319+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.004144+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.397966+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 991b806d57fc7d91b951ca4487ca6cbef011a0f30059c3379e7006a0e41ab90d |