desulfonema_magnum_medium

CultureMech:010335 bacterial COMPLEX LIQUID
Original name: Desulfonema Magnum Medium

Overview

External ontology
TOGO:M915 — Desulfonema Magnum Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M915 Original source: JCM - JCM_M876 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=876

Composition

flowchart LR
    CultureMech_010335["desulfonema_magnum_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010335 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010335 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_010335 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010335 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_010335 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_010335 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_010335 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010335 --> ing7_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_6H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_010335 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_010335 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_Nitrogen_gas["Nitrogen gas"]
    CultureMech_010335 --> ing10_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing10_Nitrogen_gas -.-> CHEBI_17997
    ing11_sodium_dithionate["sodium dithionate"]
    CultureMech_010335 --> ing11_sodium_dithionate
    ing12_N2["N2"]
    CultureMech_010335 --> ing12_N2
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
6.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
1.4 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 KH2PO4
H2O4P.K 136.084 g/mol
0.14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
6 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
7 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
8 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
5.6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
9 KCl
Cl.K 74.551 g/mol
0.72 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: ガス
11 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: ガス
12 sodium dithionate variable VARIABLE Properties: Simple component, Defined component
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: ガス

Solutions

FeCl2 solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Trace element solution (see Medium [M180])

Dose: 1 G_PER_L

Cross-reference to Medium M180

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

0.5% Na2CO3 solution

Dose: 20 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

3% Na2S・9H2O solution

Dose: 10 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

4.8% KAl(SO4)2・12H2O solution

Dose: 10 G_PER_L

Role: Mineral source; Properties: Solution, Inorganic compound, Defined component

6% Sodium benzoate solution

Dose: 10 G_PER_L

Properties: Defined component, Organic compound, Solution

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:55.955220Z togo-import Imported from TOGO Medium Source: JCM, ID: M915
2026-01-28T19:48:24.085501Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: sodium dithionate; Added default concentration for ingredient: N2
2026-03-09T08:04:39.796107+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 8 solution(s) from ingredients
2026-03-13T06:20:13.092374Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010335
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.074636+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Desulfonema Magnum Medium" to "desulfonema_magnum_medium" for consistent matching
2026-05-16T09:03:21.997570+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:45.658063+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:33.917451+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:52.032601+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:20.459212+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 426b775dc45496c6ee76a727c9a26b3b38a667b65f2a1964e1ae0794df4cd460