Overview
- External ontology
- TOGO:M927 — MMA Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M927 Original source: JCM - JCM_M886 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=886
Composition
flowchart LR
CultureMech_010348["mma_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010348 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_K2HPO4["K2HPO4"]
CultureMech_010348 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NaHCO3["NaHCO3"]
CultureMech_010348 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_010348 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_HEPES["HEPES"]
CultureMech_010348 --> ing4_HEPES
CHEBI_46756["CHEBI:46756"]
ing4_HEPES -.-> CHEBI_46756
ing5_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010348 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010348 --> ing6_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_2H2O -.-> CHEBI_86158
ing7_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010348 --> ing7_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_2H2O -.-> CHEBI_75213
ing8_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010348 --> ing8_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing8_CoCl2_6H2O -.-> CHEBI_35696
ing9_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010348 --> ing9_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_7H2O -.-> CHEBI_32312
ing10_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010348 --> ing10_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_5H2O -.-> CHEBI_31440
ing11_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010348 --> ing11_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing11_MnSO4_xH2O -.-> CHEBI_86360
ing12_EDTA_Fe_III_["EDTA・Fe(III)"]
CultureMech_010348 --> ing12_EDTA_Fe_III_
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 5 | HEPES | 11.9 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 6 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
5 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 10 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.01 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 11 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 | EDTA・Fe(III) | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component |
Solutions
Trace element solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M927_MMA_Medium (CultureMech:010348)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.975769Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M927 |
| 2026-01-28T19:48:25.333803Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:41.902999+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:11.908220Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:20:13.182949Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010348 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.162628+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMA Medium" to "mma_medium" for consistent matching |
| 2026-05-16T09:03:22.085999+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.761008+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:34.006930+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.077005+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.550373+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 25365aefee612489275b8b86b565c38267cc8f51f7c4461375267dd12785ac93 |