Overview
- External ontology
- TOGO:M928 — Natronolimnobius AHT32 Medium (A)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M928 Original source: JCM - JCM_M887 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=887
Composition
flowchart LR
CultureMech_010349["natronolimnobius_aht32_medium_a"]
ing0_Distilled_water["Distilled water"]
CultureMech_010349 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010349 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_K2HPO4["K2HPO4"]
CultureMech_010349 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_NaHCO3["NaHCO3"]
CultureMech_010349 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_Na2CO3["Na2CO3"]
CultureMech_010349 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_1_M_Sodium_acetate["1 M Sodium acetate"]
CultureMech_010349 --> ing5_1_M_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing5_1_M_Sodium_acetate -.-> CHEBI_32954
ing6_1_M_Sodium_butyrate["1 M Sodium butyrate"]
CultureMech_010349 --> ing6_1_M_Sodium_butyrate
CHEBI_64103["CHEBI:64103"]
ing6_1_M_Sodium_butyrate -.-> CHEBI_64103
ing7_Nitrogen_gas["Nitrogen gas"]
CultureMech_010349 --> ing7_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing7_Nitrogen_gas -.-> CHEBI_17997
ing8_Oxygen_gas["Oxygen gas"]
CultureMech_010349 --> ing8_Oxygen_gas
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
16 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
190 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
1 M Sodium acetate
C2H3O2.Na 82.034 g/mol
|
20 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Solution, Defined component |
| 7 |
1 M Sodium butyrate
C4H7O2.Na 110.088 g/mol
|
20 G_PER_L |
CHEBI:64103
sodium butyrate
|
Properties: Defined component, Organic compound, Solution |
| 8 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 9 | Oxygen gas | variable VARIABLE | Properties: ガス |
Solutions
10% Yeast extract solution
Dose: 0.2 G_PER_L
Role: Nutrient source; Properties: Undefined component
4 M NH4Cl solution
Dose: 1 G_PER_L
Role: Nitrogen source; Properties: Defined component, Solution, Inorganic compound
1 M MgSO4 solution
Dose: 1 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
SL--4 trace element solution (see Medium [M335])
Dose: 1 G_PER_L
Cross-reference to Medium M335
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M928_Natronolimnobius_AHT32_Medium_A (CultureMech:010349)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.977757Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M928 |
| 2026-01-28T19:48:27.449123Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:04:45.466765+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T06:20:13.191559Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010349 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.171367+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Natronolimnobius AHT32 Medium (A)" to "natronolimnobius_aht32_medium_a" for consistent matching |
| 2026-05-16T09:03:22.095845+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.770925+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.081351+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:27.898339+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 16c810932989edbff23c9b18f2ec0736553ec708fd0aa9f861510ed28b2ea146 |