Overview
- External ontology
- TOGO:M934 — Mbbs Medium For Hydrogenobacter
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M934 Original source: JCM - JCM_M893 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=893
Composition
flowchart LR
CultureMech_010356["mbbs_medium_for_hydrogenobacter"]
ing0_Sulfur__powder_["Sulfur (powder)"]
CultureMech_010356 --> ing0_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing0_Sulfur__powder_ -.-> CHEBI_33403
ing1_H2SO4["H2SO4"]
CultureMech_010356 --> ing1_H2SO4
CHEBI_26836["CHEBI:26836"]
ing1_H2SO4 -.-> CHEBI_26836
ing2_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010356 --> ing2_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing2_Carbon_dioxide_gas -.-> CHEBI_16526
ing3_Oxygen_gas["Oxygen gas"]
CultureMech_010356 --> ing3_Oxygen_gas
ing4_Hydrogen_gas["Hydrogen gas"]
CultureMech_010356 --> ing4_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing4_Hydrogen_gas -.-> CHEBI_18276
ing5_Distilled_water["Distilled water"]
CultureMech_010356 --> ing5_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing5_Distilled_water -.-> CHEBI_15377
ing6_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010356 --> ing6_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_7H2O -.-> CHEBI_31795
ing7_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010356 --> ing7_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_2H2O -.-> CHEBI_86158
ing8_KH2PO4["KH2PO4"]
CultureMech_010356 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010356 --> ing9_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_2H2O -.-> CHEBI_75213
ing10_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010356 --> ing10_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_4H2O -.-> CHEBI_86368
ing11_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010356 --> ing11_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_7H2O -.-> CHEBI_32312
ing12_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_010356 --> ing12_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_2H2O -.-> CHEBI_86318
ing13__NH4_2SO4["(NH4)2SO4"]
CultureMech_010356 --> ing13__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing13__NH4_2SO4 -.-> CHEBI_62946
ing14_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_010356 --> ing14_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing14_FeCl3_6H2O -.-> CHEBI_86254
ing15_CoSO4_7H2O["CoSO4・7H2O"]
CultureMech_010356 --> ing15_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_7H2O -.-> CHEBI_91244
ing16_VOSO4_xH2O["VOSO4・xH2O"]
CultureMech_010356 --> ing16_VOSO4_xH2O
ing17_Na2B4O7_10H2O["Na2B4O7・10H2O"]
CultureMech_010356 --> ing17_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing17_Na2B4O7_10H2O -.-> CHEBI_131366
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sulfur (powder) | 1 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 3 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 4 | Oxygen gas | variable VARIABLE | Properties: ガス | |
| 5 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 6 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
2 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoSO4・7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.01 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 | VOSO4・xH2O | 0.03 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 18 |
Na2B4O7・10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
4.5 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
8% NaHCO3 solution (filter--sterilized)
Dose: 10 G_PER_L
Role: Buffer; Properties: Inorganic compound, Defined component, Solution
10% Yeast extract solution
Dose: 5 G_PER_L
Role: Nutrient source; Properties: Undefined component
Modified Brock's salt base solution (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M934_Mbbs_Medium_For_Hydrogenobacter (CultureMech:010356)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:55.988488Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M934 |
| 2026-01-28T19:48:34.553016Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Oxygen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:57.387897+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:20:13.237358Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010356 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.215319+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mbbs Medium For Hydrogenobacter" to "mbbs_medium_for_hydrogenobacter" for consistent matching |
| 2026-05-16T09:03:22.139497+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:45.822667+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:34.054374+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.093149+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.605155+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 77aee459d7cdee84e7a1ca934f50332d7b8c76e1d2cacfe964d8d4551f6d84c8 |