Overview
- External ontology
- TOGO:M937 — Modified BSK Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M937 Original source: JCM - JCM_M896 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=896
Composition
flowchart LR
CultureMech_010359["modified_bsk_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010359 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaHCO3["NaHCO3"]
CultureMech_010359 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_010359 --> ing2_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Sodium_pyruvate -.-> CHEBI_50144
ing3_HEPES["HEPES"]
CultureMech_010359 --> ing3_HEPES
CHEBI_46756["CHEBI:46756"]
ing3_HEPES -.-> CHEBI_46756
ing4_N_Acetyl_D_glucosamine["N-Acetyl-D-glucosamine"]
CultureMech_010359 --> ing4_N_Acetyl_D_glucosamine
CHEBI_8006["CHEBI:8006"]
ing4_N_Acetyl_D_glucosamine -.-> CHEBI_8006
ing5_Probumin_Universal_Grade__Millipore_["Probumin Universal Grade (Millipore)"]
CultureMech_010359 --> ing5_Probumin_Universal_Grade__Millipore_
ing6_TC_Yeastolate__BD_Difco_["TC Yeastolate (BD-Difco)"]
CultureMech_010359 --> ing6_TC_Yeastolate__BD_Difco_
ing7_Rabbit_serum_sterile_non__hemolyzed_young__Pel__Freez_code_No__31125_["Rabbit serum sterile non--hemolyzed young (Pel--Freez code No. 31125)"]
CultureMech_010359 --> ing7_Rabbit_serum_sterile_non__hemolyzed_young__Pel__Freez_code_No__31125_
ing8_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
CultureMech_010359 --> ing8_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing8_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing9_Glucose["Glucose"]
CultureMech_010359 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_MEM_alpha_Modification__BioWest_["MEM alpha Modification (BioWest)"]
CultureMech_010359 --> ing10_MEM_alpha_Modification__BioWest_
ing11_Neopeptone__BD_Difco_["Neopeptone (BD-Difco)"]
CultureMech_010359 --> ing11_Neopeptone__BD_Difco_
ing12_NaOH["NaOH"]
CultureMech_010359 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
584 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.4 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 4 | HEPES | 3 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 5 |
N-Acetyl-D-glucosamine
C34H60N8O18 868.892 g/mol
|
0.2 G_PER_L |
CHEBI:8006
Peptidoglycan(N-acetyl-D-glucosamine)
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 6 | Probumin Universal Grade (Millipore) | 25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 7 | TC Yeastolate (BD-Difco) | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 8 | Rabbit serum sterile non--hemolyzed young (Pel--Freez code No. 31125) | 40 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 9 |
Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
|
0.35 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 11 | MEM alpha Modification (BioWest) | 5.2 G_PER_L | Properties: Defined component, Complex component | |
| 12 | Neopeptone (BD-Difco) | 2.5 G_PER_L | Properties: Undefined component | |
| 13 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M937_Modified_BSK_Medium (CultureMech:010359)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:55.992376Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M937 |
| 2026-01-28T19:48:12.489747Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-13T06:20:13.253996Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010359 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.232430+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified BSK Medium" to "modified_bsk_medium" for consistent matching |
| 2026-05-16T09:03:22.154149+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.838336+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:20.625753+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2f0347817b6183c19d532f4ad6a0cbb2c8a219c0288d1027134eb5e24ceef9f9 |