Overview
- External ontology
- TOGO:M949 — MK Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M949 Original source: JCM - JCM_M906 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=906
Composition
flowchart LR
CultureMech_010372["mk_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010372 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010372 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KH2PO4["KH2PO4"]
CultureMech_010372 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_010372 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010372 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010372 --> ing5_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_4H2O -.-> CHEBI_86368
ing6_K2SO4["K2SO4"]
CultureMech_010372 --> ing6_K2SO4
CHEBI_32036["CHEBI:32036"]
ing6_K2SO4 -.-> CHEBI_32036
ing7_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_010372 --> ing7_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_4H2O -.-> CHEBI_86249
ing8_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
CultureMech_010372 --> ing8_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing8_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing9_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
CultureMech_010372 --> ing9_Tryptone__BD_Difco_
ing10_Peptone__Oxoid_["Peptone (Oxoid)"]
CultureMech_010372 --> ing10_Peptone__Oxoid_
ing11_0_5_M_Glycine__NaOH_buffer__pH_10_0_["0.5 M Glycine--NaOH buffer (pH 10.0)"]
CultureMech_010372 --> ing11_0_5_M_Glycine__NaOH_buffer__pH_10_0_
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
900 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.6 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
K2SO4
2K.O4S 174.259 g/mol
|
20 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
|
1 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 10 | Tryptone (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 11 | Peptone (Oxoid) | 1 G_PER_L | Properties: Undefined component | |
| 12 | 0.5 M Glycine--NaOH buffer (pH 10.0) | 100 G_PER_L | Role: Buffer; Properties: Solution, Defined component, Complex component |
Solutions
SL-6 trace element solution (see Medium [M158])
Dose: 1 G_PER_L
Cross-reference to Medium M158
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M949_MK_Medium (CultureMech:010372)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.017311Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M949 |
| 2026-01-28T19:48:24.483058Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.474627+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:20:13.358765Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010372 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.336818+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MK Medium" to "mk_medium" for consistent matching |
| 2026-05-16T09:03:22.265632+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:45.965713+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.131493+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.763729+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 913f0d517c25582210974dc9dd058e38a8e7c581ab3a77bc597bffc454caee72 |