desulfamplus_medium

CultureMech:010389 bacterial COMPLEX LIQUID
Original name: Desulfamplus Medium

Overview

External ontology
TOGO:M964 — Desulfamplus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M964 Original source: JCM - JCM_M918 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=918

Composition

flowchart LR
    CultureMech_010389["desulfamplus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010389 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_010389 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_010389 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_010389 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_010389 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_010389 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_010389 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_010389 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_Na2SO4["Na2SO4"]
    CultureMech_010389 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_HEPES["HEPES"]
    CultureMech_010389 --> ing9_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing9_HEPES -.-> CHEBI_46756
    ing10_Sodium_fumarate["Sodium fumarate"]
    CultureMech_010389 --> ing10_Sodium_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing10_Sodium_fumarate -.-> CHEBI_115156
    ing11_NaOH["NaOH"]
    CultureMech_010389 --> ing11_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing11_NaOH -.-> CHEBI_32145
    ing12_N2["N2"]
    CultureMech_010389 --> ing12_N2
    CHEBI_17997["CHEBI:17997"]
    ing12_N2 -.-> CHEBI_17997
    ing13_0_5_M_Potassium_phosphate_buffer__pH_7_0_["0.5 M Potassium phosphate buffer (pH 7.0)"]
    CultureMech_010389 --> ing13_0_5_M_Potassium_phosphate_buffer__pH_7_0_
    ing14_HCl["HCl"]
    CultureMech_010389 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 0.2 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 NH4Cl
Cl.H4N 53.492 g/mol
0.3 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
6 Resazurin
C12H7NO4 229.191 g/mol
0.4 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8 KCl
Cl.K 74.551 g/mol
0.55 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
9 Na2SO4
2Na.O4S 142.043 g/mol
3.24 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
10 HEPES 2.4 G_PER_L CHEBI:46756
HEPES
 Role: Buffer; Properties: Simple component, Organic compound, Defined component
11 Sodium fumarate
C4H2O4.2Na 160.036 g/mol
1 G_PER_L CHEBI:115156
disodium fumarate
 Properties: Defined component, Organic compound, Simple component
12 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
14 0.5 M Potassium phosphate buffer (pH 7.0) 1.8 G_PER_L Role: Buffer; Properties: Solution, Defined component, Inorganic compound, Complex component
15 HCl
HCl 36.461 g/mol
variable VARIABLE CHEBI:17883
hydrogen chloride
 Role: Solvating media; Properties: Defined component, Inorganic compound, Solution

Solutions

Modified Wolfe's mineral solution (see Medium [M961])

Dose: 5 G_PER_L

Cross-reference to Medium M961

8% NaHCO3 solution*

Dose: 2.35 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

5% L--Cysteine・HCl・H2O solution (pH 7.0)

Dose: 8 G_PER_L

Properties: Defined component, Organic compound, Solution

0.01 M FeSO4・5H2Osolution (in 0.02 N HCl) (optional)

Dose: 10 G_PER_L

Role: Mineral source; Properties: Defined component, Solution, Inorganic compound

Vitamin solution (see Medium [M961])

Dose: 0.5 G_PER_L

Cross-reference to Medium M961

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:56.045549Z togo-import Imported from TOGO Medium Source: JCM, ID: M964
2026-01-28T19:48:36.344435Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: HCl
2026-03-09T08:05:00.602853+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T06:20:13.486699Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010389
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.460796+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Desulfamplus Medium" to "desulfamplus_medium" for consistent matching
2026-05-16T09:03:22.374695+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:46.090575+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.170430+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:20.868196+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 9fe3128487542a177793254a9c1de70ecda2396b91d1e62ae4bd19a7d79a8de3