Overview
- External ontology
- TOGO:M973 — Desulfatiferula Berrense Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M973 Original source: JCM - JCM_M927 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=927
Composition
flowchart LR
CultureMech_010399["desulfatiferula_berrense_medium"]
ing0_Substrate["Substrate"]
CultureMech_010399 --> ing0_Substrate
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010399 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_Nitrogen_gas["Nitrogen gas"]
CultureMech_010399 --> ing2_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing2_Nitrogen_gas -.-> CHEBI_17997
ing3_Distilled_water["Distilled water"]
CultureMech_010399 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
ing4_NaCl["NaCl"]
CultureMech_010399 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010399 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_KH2PO4["KH2PO4"]
CultureMech_010399 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NH4Cl["NH4Cl"]
CultureMech_010399 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Resazurin["Resazurin"]
CultureMech_010399 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_KCl["KCl"]
CultureMech_010399 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_Na2SO4["Na2SO4"]
CultureMech_010399 --> ing10_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing10_Na2SO4 -.-> CHEBI_32149
ing11_HEPES["HEPES"]
CultureMech_010399 --> ing11_HEPES
CHEBI_46756["CHEBI:46756"]
ing11_HEPES -.-> CHEBI_46756
ing12_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_010399 --> ing12_Yeast_extract__BD_Difco_
ing13_N2["N2"]
CultureMech_010399 --> ing13_N2
ing13_N2 -.-> CHEBI_17997
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Substrate | 20 G_PER_L | ||
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 3 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 4 |
Distilled water
H2O 18.015 g/mol
|
960 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
7 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 12 | HEPES | 2.38 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 13 | Yeast extract (BD-Difco) | 0.1 G_PER_L | Properties: Undefined component | |
| 14 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Na2S・9H2O solutions
Dose: 0.01 PERCENT_W_V
Role: Reducing agent; Properties: Inorganic compound, Solution, Defined component
Solution A
Dose: 962 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Trace metal solution SL12 (see Medium [M581])
Dose: 1 G_PER_L
Cross-reference to Medium M581
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
8% NaHCO3 solution
Dose: 30 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
15% MgCl2・6H2O solution*
Dose: 10 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
Vitamin solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Thiamine solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
Vitamin B12 solution (see Medium [M401])
Dose: 1 G_PER_L
Cross-reference to Medium M401
sodium octanoate solution
Dose: 20 G_PER_L
Role: Carbon source; Properties: Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M973_Desulfatiferula_Berrense_Medium (CultureMech:010399)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.062495Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M973 |
| 2026-01-28T19:48:14.695362Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:24.426631+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 10 solution(s) from ingredients |
| 2026-03-13T06:20:13.562642Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010399 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.535479+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfatiferula Berrense Medium" to "desulfatiferula_berrense_medium" for consistent matching |
| 2026-05-16T09:03:22.446001+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.170924+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.195055+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.947122+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2c59aa6809d7994e5f8037585aae75f4932a8d518bb0e8db7770d3eab1c3a295 |