Overview
- External ontology
- TOGO:M974 — Methylobacter MR1 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M974 Original source: JCM - JCM_M928 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=928
Composition
flowchart LR
CultureMech_010400["methylobacter_mr1_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_010400 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_010400 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_010400 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010400 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_010400 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_010400 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010400 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_FeSO4_7H2O["FeSO4・7H2O"]
CultureMech_010400 --> ing7_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_7H2O -.-> CHEBI_75836
ing8_KCl["KCl"]
CultureMech_010400 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaNO3["NaNO3"]
CultureMech_010400 --> ing9_NaNO3
CHEBI_63005["CHEBI:63005"]
ing9_NaNO3 -.-> CHEBI_63005
ing10_Na2HPO4["Na2HPO4"]
CultureMech_010400 --> ing10_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing10_Na2HPO4 -.-> CHEBI_34683
ing11_N2["N2"]
CultureMech_010400 --> ing11_N2
CHEBI_17997["CHEBI:17997"]
ing11_N2 -.-> CHEBI_17997
ing12_O2["O2"]
CultureMech_010400 --> ing12_O2
ing13_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010400 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_010400 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010400 --> ing15_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_6H2O -.-> CHEBI_34887
ing16_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010400 --> ing16_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_7H2O -.-> CHEBI_32312
ing17_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_010400 --> ing17_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing17_Na2SeO3_5H2O -.-> CHEBI_131361
ing18_CoSO4_7H2O["CoSO4・7H2O"]
CultureMech_010400 --> ing18_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing18_CoSO4_7H2O -.-> CHEBI_91244
ing19_AlK_SO4_2_12H2O["AlK(SO4)2・12H2O"]
CultureMech_010400 --> ing19_AlK_SO4_2_12H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_12H2O -.-> CHEBI_86465
ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_010400 --> ing20_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
ing21_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_010400 --> ing21_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_2H2O -.-> CHEBI_63939
ing22_MnSO4_xH2O["MnSO4・xH2O"]
CultureMech_010400 --> ing22_MnSO4_xH2O
CHEBI_86360["CHEBI:86360"]
ing22_MnSO4_xH2O -.-> CHEBI_86360
ing23_KOH["KOH"]
CultureMech_010400 --> ing23_KOH
CHEBI_32035["CHEBI:32035"]
ing23_KOH -.-> CHEBI_32035
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.09 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.12000000000000001 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.02, 0.1] |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaNO3
NO3.Na 84.994 g/mol
|
0.25 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.26 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 | O2 | variable VARIABLE | Properties: ガス | |
| 14 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
5 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CoSO4・7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
AlK(SO4)2・12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 22 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.02 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
MnSO4・xH2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
1 mM CuSO4・5H2O solution
Dose: 0.1 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Inorganic compound
Trace mineral solution (see below)
Dose: 3 G_PER_L
8% NaHCO3 solution
Dose: 1.25 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 1 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M974_Methylobacter_MR1_Medium (CultureMech:010400)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.064973Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M974 |
| 2026-01-28T19:48:36.439984Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2; Added default concentration for ingredient: O2; Added default concentration for ingredient: KOH |
| 2026-03-09T08:05:00.752441+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T03:09:32.573808Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:20:13.575769Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010400 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.547951+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methylobacter MR1 Medium" to "methylobacter_mr1_medium" for consistent matching |
| 2026-05-16T09:03:22.460363+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.185747+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:34.346025+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.201377+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:20.965207+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6e8c0c5234821d3c13a33e68e684662f1a9374faede80bc99b1fcf638175a2e8 |