modified_halosimplex_medium

CultureMech:010413 archaea COMPLEX SOLID_AGAR
Original name: Modified Halosimplex Medium

Overview

External ontology
TOGO:M986 — Modified Halosimplex Medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'halosimplex_medium', 'source': 'TOGO', 'source_id': 'TOGO:M293', 'original_category': 'archaea'}
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M986 Original source: JCM - JCM_M939-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=939

Composition

flowchart LR
    CultureMech_010413["modified_halosimplex_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_010413 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_010413 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_010413 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_K2HPO4["K2HPO4"]
    CultureMech_010413 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_KCl["KCl"]
    CultureMech_010413 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_010413 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_010413 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144
    ing7_Glycerol["Glycerol"]
    CultureMech_010413 --> ing7_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing7_Glycerol -.-> CHEBI_17754
    ing8_Bacto_agar__BD_Difco_["Bacto agar (BD-Difco)"]
    CultureMech_010413 --> ing8_Bacto_agar__BD_Difco_

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 NaCl
Cl.Na 58.443 g/mol
200 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 K2HPO4
HO4P.2K 174.174 g/mol
0.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
5 KCl
Cl.K 74.551 g/mol
4 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
7 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
2.5 G_PER_L CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
8 Glycerol
C3H8O3 92.094 g/mol
2.5 G_PER_L CHEBI:17754
glycerol
 Role: Carbon source; Properties: Simple component, Organic compound, Defined component
9 Bacto agar (BD-Difco) 20 G_PER_L Role: Solidifying component

Solutions

Trace metal solution (see Medium [M292])

Dose: 2 G_PER_L

Cross-reference to Medium M292

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:56.087865Z togo-import Imported from TOGO Medium Source: JCM, ID: M986
2026-01-28T19:48:29.638181Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:49.238133+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:20:13.673539Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:010413
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:28.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:32.650514+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Halosimplex Medium" to "modified_halosimplex_medium" for consistent matching
2026-05-16T09:03:22.546659+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:46.280031+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:35.333184+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:34.412481+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:52.235184+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:27.327626+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: TOGO_M293_Halosimplex_Medium, TOGO_M986_Modified_Halosimplex_Medium
2026-08-06T03:04:27.327669+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 3d1e78106e0c81e8e0b3a2d66bc9187bca20b76053ab552dbdbb86f43f6e835c