Overview
- External ontology
- TOGO:M991 — MW Medium For Methanosarcina
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M991 Original source: JCM - JCM_M944 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=944
Composition
flowchart LR
CultureMech_010419["mw_medium_for_methanosarcina"]
ing0_Distilled_water["Distilled water"]
CultureMech_010419 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_010419 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_010419 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_010419 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Resazurin["Resazurin"]
CultureMech_010419 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_010419 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_010419 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_010419 --> ing7_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing7_Carbon_dioxide_gas -.-> CHEBI_16526
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_010419 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_N2["N2"]
CultureMech_010419 --> ing9_N2
ing9_N2 -.-> CHEBI_17997
ing10_Hydrogen_gas["Hydrogen gas"]
CultureMech_010419 --> ing10_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing10_Hydrogen_gas -.-> CHEBI_18276
ing11_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_010419 --> ing11_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_2H2O -.-> CHEBI_75213
ing12_H3BO3["H3BO3"]
CultureMech_010419 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_010419 --> ing13_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_4H2O -.-> CHEBI_86368
ing14_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_010419 --> ing14_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing14_CoCl2_6H2O -.-> CHEBI_35696
ing15_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_010419 --> ing15_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_6H2O -.-> CHEBI_34887
ing16_ZnSO4_7H2O["ZnSO4・7H2O"]
CultureMech_010419 --> ing16_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_7H2O -.-> CHEBI_32312
ing17_CuSO4_5H2O["CuSO4・5H2O"]
CultureMech_010419 --> ing17_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_5H2O -.-> CHEBI_31440
ing18_Na2SeO3_5H2O["Na2SeO3・5H2O"]
CultureMech_010419 --> ing18_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_5H2O -.-> CHEBI_131361
ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_010419 --> ing19_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
ing20_Na2WO4_2H2O["Na2WO4・2H2O"]
CultureMech_010419 --> ing20_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_2H2O -.-> CHEBI_63939
ing21_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2・6H2O"]
CultureMech_010419 --> ing21_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing21_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing22_KOH["KOH"]
CultureMech_010419 --> ing22_KOH
CHEBI_32035["CHEBI:32035"]
ing22_KOH -.-> CHEBI_32035
ing23_Biotin["Biotin"]
CultureMech_010419 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_p__Aminobenzoic_acid["p--Aminobenzoic acid"]
CultureMech_010419 --> ing24_p__Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing24_p__Aminobenzoic_acid -.-> CHEBI_30753
ing25_Thiamine_HCl["Thiamine・HCl"]
CultureMech_010419 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_010419 --> ing26_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_HCl -.-> CHEBI_30961
ing27_Folic_acid["Folic acid"]
CultureMech_010419 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_010419 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_Riboflavin["Riboflavin"]
CultureMech_010419 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_010419 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
948.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 946.0, 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 12 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.11 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
0.1 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
0.25 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
ZnSO4・7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CuSO4・5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.002 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2SeO3・5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.2 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 21 |
Na2WO4・2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Fe(NH4)2(SO4)2・6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.2 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
5 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
p--Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
10 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
10 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 27 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
5 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 | Vitamin B12 | 5 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
10 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
10 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 33 | DL--Calcium pantothenate | 10 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace elements solution (see below)
Dose: 10 G_PER_L
8% NaHCO3 solution*
Dose: 32 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
10% KH2PO4 solution
Dose: 2 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Vitamin solution* (see below)
Dose: 10 G_PER_L
5% Na2S・9H2O solution
Dose: 6 G_PER_L
Role: Reducing agent; Properties: Solution, Inorganic compound, Defined component
5% L--Cysteine・HCl・H2O solution
Dose: 6 G_PER_L
Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M991_MW_Medium_For_Methanosarcina (CultureMech:010419)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:56.098102Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M991 |
| 2026-01-28T19:48:20.306381Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:33.655878+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 6 solution(s) from ingredients |
| 2026-03-13T03:09:03.138348Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:20:13.726865Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:010419 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:32.703063+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MW Medium For Methanosarcina" to "mw_medium_for_methanosarcina" for consistent matching |
| 2026-05-16T09:03:22.607215+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:46.347398+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.349298+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:52.253468+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.017252+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 989c029818597dbdeb592727f0c15579eebbb0c6bba60f9f075fbec076b26fa9 |